摘要
目的:优化采用大孔吸附树脂分离、纯化返魂草有效成分的工艺。方法:以返魂草中有效成分绿原酸和金丝桃苷的吸附量、解析量等为考察指标,通过静态吸附选择适合的大孔吸附树脂型号;以绿原酸和金丝桃苷的洗脱量等为指标,采用动态吸附法确定最大上样量、水洗量、洗脱剂乙醇的体积分数及收集量等最优的分离、纯化条件。结果:7种型号树脂中以HPD100树脂纯化性能最好;在上样液质量浓度为6 mg/ml、上样速度为2 ml/min时,以最大上样量为3倍柱体积(BV)、水洗量为2.5 BV、60%乙醇洗脱并收集3 BV为最优条件。纯化后提取物中绿原酸和金丝桃苷含量由0.90、0.18 mg/g增加到7.26、1.04 mg/g;验证试验中各指标的RSD均≤3.0%(n=3)。结论:建立的以HPD100大孔吸附树脂分离、纯化返魂草中有效成分的工艺稳定、高效。
OBJECTIVE：To optimize the isolation and purification technology of active components of Senecio cannabifolius with macroporous adsorption resin. METHODS：The type of macroporous adsorption resin was selected with static adsorption using adsorption capacity and resolution rate of chlorogenic acid and hyperoside as index. The isolation and purification condition was optimized with dynamic adsorption using the elution volume of chlorogenic acid and hyperoside as index,such as maximal sample size,rinse water quantity,volume fraction and collective volume of eluant ethanol. RESULTS：Among 7 kinds of resin,HPD100 had the best purification property;the optimal purification technology was as follows as mass concentration of sample 6 mg/ml,the speed of sample loading 2 ml/min,maximal sample size 3 times of column volume（BV）, rinse water quantity of 2.5 BV,60%ethanol as eluting reagent. The contents of chlorogenic acid and hyperoside were increased from 0.90,0.18 mg/g to 7.26,1.04 mg/g after purification. RSD of each index were all ≤3.0%（n=3） in validation test. CONCLUSIONS：The isolation and purification technology of active components of S. cannabifolius with HPD100 macroporous adsorption resin is stable and effective.
出处
《中国药房》
CAS
北大核心
2015年第31期4402-4405,共4页
China Pharmacy
基金
吉林省教育厅"十二五"科学技术研究项目(No.吉教科合字〔2014〕第549号)
关键词
大孔吸附树脂
返魂草
绿原酸
金丝桃苷
分离
纯化
Macroporous adsorption resin
Senecio cannabifolius
Chlorogenic acid
Hyperoside
Isolation
Purification
