摘要
目的 建立同时检测人血浆中利多卡因和丁卡因的方法。方法 采用 RP- HPL C法测定人血浆中药物浓度 ,色谱柱为 L ichrospher C18(4.6 mm× 2 5 0 mm,5μm) ,流动相为甲醇 -水 -冰醋酸 -三乙胺 (6 2∶ 38∶ 0 .3∶0 .6 )。结果 在 0 .1～ 8mg· L-1范围内利多卡因、丁卡因峰面积与内标峰面积之比与浓度线性关系良好 ,最低检测浓度为 0 .0 3mg· L-1,回收率利多卡因为 92 .39%～ 97.75 % ,丁卡因为 89.2 9%～ 95 .5 4% ,日内、日间RSD均小于 10 %。结论 本测定方法灵敏、准确、简便 ,适合于临床治疗药物监测及药代动力学研究。
Objective To develop a simple,sensitive and rapid method for the determination of lidocaine and tetracaine in human plasma. Method The concentration of lidocaine and tetracaine was determined by RP-HPLC method. The analysis involved a 250 mm×4.6 mm column packed with C 18(5 μm), the mobile phase consisted of methyl alcohol-water-glacial acetic acid-triethylamine(62∶38∶0.3∶0.6). Results A good linearity was obtained in the range of 0.1 mg·L -1～8 mg·L -1, the recoveries of lidocaine and tetracaine were between 92.39%～97.75% and 89.29%～95.54% respeclively, the relative standard deviations of within-day and between-day were less than 10%, andtheminimaldetectableconcentrationinplasmawas0.03 mg·L -1. Conclusion The results demonstrated that the method was sensitive, accurate and simple for the determination of lidocaine and tetracaine in human plasma, and it is suitable for the pharmacokinetics study and clinical therapeutic monitoring of lidocaine and tetracaine.
出处
《西北药学杂志》
CAS
2004年第5期195-197,共3页
Northwest Pharmaceutical Journal
