摘要
以12个风信子(Hyacinthus orientalis L.)品种为研究材料,采用英国皇家园艺学会比色卡进行花色描述,利用特征颜色反应确定色素类型,利用UPLC-PAD结合UPLC-Q-TOF-MS技术分析花色苷种类及含量。结果表明,12个品种花瓣中均不含有色的类胡萝卜素,除‘City of Haarlem’和‘Aiolos’以外,均含花色苷。10个含花色苷的品种中,‘Woodstock’花瓣中花色苷含量最高,‘Jan Bos’、‘上农早粉’、‘上农紫红’次之。在这些品种花瓣中共检测到9种花色苷成分,通过与已有文献比对,推定其成分为:天竺葵素3–O–葡萄糖苷、天竺葵素3–O–葡萄糖苷5–O–丙二酰葡萄糖苷、矢车菊素3–O–香豆酰葡萄糖苷–5–O–葡萄糖苷、天竺葵素3–O–香豆酰葡萄糖苷–5–O–葡萄糖苷、天竺葵素3–O–香豆酰葡萄糖苷–5–O–乙酰葡萄糖苷、矢车菊素3–O–香豆酰葡萄糖苷–5–O–丙二酰葡萄糖苷、天竺葵素3–O–香豆酰葡萄糖苷–5–O–丙二酰葡萄糖苷、天竺葵素3–O–阿魏酰葡萄糖苷–5–O–丙二酰葡萄糖苷和天竺葵素3–O–咖啡酰葡萄糖苷–5–O–丙二酰葡萄糖苷。‘上农早粉’、‘上农中粉’、‘Gipsy Queen’、‘Marconi’等品种花瓣中仅含天竺葵素花色苷衍生物,其它6个品种则含天竺葵素和矢车菊素的花色苷衍生物,以天竺葵素为主。‘上农早粉’、‘上农中粉’、‘Gipsy Queen’、‘Jan Bos’、‘Marconi’和‘Lady Derby’花瓣花色苷以天竺葵素3–O–香豆酰葡萄糖苷–5–O–丙二酰葡萄糖苷为主;‘Anna Lisa’、‘Woodstock’花瓣花色苷以天竺葵素3–O–香豆酰葡萄糖苷–5–O–乙酰葡萄糖苷为主;‘上农紫红’和‘Sky Line’花瓣花色苷分别以矢车菊素3–O–香豆酰葡萄糖苷–5–O–丙二酰葡萄糖苷以及天竺葵素3–O–香豆酰葡萄糖苷–5–O–葡萄糖苷为主。
The anthocyanin profiles in 12 cultivars of hyacinthus(Hyacinthus orientalis L.)were analyzed. The flower color was observed according to the Royal Horticultural Society Color Card(RHSCC). Petal pigment type was identified by characteristic color reaction. UPLC-PAD and UPLC-Q-TOF-MS were used for qualitative and quantitative analysis of anthocyanins. The results showed that colored carotenoids in petals of all cultivars were not detected,by contrast,every cultivar,except for ‘City of Haarlem'and‘Aiolos',contained anthocyanins in petals. Among ten anthocyanin-contained cultivars,‘Woodstock'contained the highest anthocyanin content,followed by‘Jan Bos',‘Shangnong Zaofen',and‘Shangnong Zihong',respectively. Totally 9 anthocyanin components were detected in petals of these cultivars,including Pelargonidin 3-O-glucoside,Pelargonidin 3-O-glucoside-5-O-malonylglucoside,Cyanidin 3-O-coumaroylglucoside-5-O-glucoside,Pelargonidin 3-O-coumaroyl-glucoside-5-O-glucoside,Pelargonidin 3-O-coumaroylglucoside-5-O-acetyglucoside,Cyanidin 3-O-coumaroylglucoside-5-Omalonylglucoside,Pelargonidin 3-O-coumaroylglucoside-5-O-malonylglucoside,Pelargonidin3-Oferuloylglucoside-5-O-malonylglucoside,and Pelargonidin 3-O-caffeoylglucoside-5-O-malonylglucoside.‘Shangnong Zaofen',‘Shangnong Zhongfen',‘Gipsy Queen',and‘Marconi'contained only pelargonidin derivated anthocyanins in petals while other six cultivars contained majorly pelargonidin derivated anthocyanins and some cyanidin derivated anthocyanins in petals. Pelargonidin 3-Ocoumaroylglucoside-5-O-malonylglucoside was the dominant anthocyanin in ‘ Shangnong Zaofen ',‘Shangnong Zhongfen',‘Gipsy Queen',‘Jan Bos',‘Marconi',and‘Lady Derby',while Pelargonidin 3-O-coumaroylglucoside-5-O-acetyglucoside was the dominant anthocyanin in ‘ Anna Lisa ' and ‘ Woodstock '; While, Cyanidin 3-O-coumaroylglucoside-5-O-malonylglucoside and Pelargonidin 3-O-coumaroylglucoside-5-O-glucoside was the dominant anthocyanin in‘Shangnong Zihong'and‘Sky Line',respectively.
出处
《园艺学报》
CAS
CSCD
北大核心
2015年第2期301-310,共10页
Acta Horticulturae Sinica
基金
上海市科学委员会项目(13391901002)
上海市农业委员会项目[沪农科攻字(2014)第1-2号]
关键词
风信子
花色
花色苷
液质联用
Hyacinthus orientalis
flower color
anthocyanin
UPLC-MS
