摘要
采用高效液相色谱—质谱法对芸薹属中红叶芥菜、紫红色大白菜、红菜薹、紫色白菜和紫结球甘蓝新鲜叶片所含花青素苷含量和组成进行鉴定。结果表明,这些蔬菜中花青素苷主要组分为矢车菊素苷,兼有少量的飞燕草素苷和矮牵牛素苷。红叶芥菜、紫红色大白菜、紫色白菜和红菜薹中均含有矢车菊–3–p–香豆酰–丙二酰–葡萄糖苷–5–葡萄糖苷、矢车菊–3–阿魏酰–丙二酰–槐糖苷–5–葡萄糖苷和矢车菊–3–芥子酰–阿魏酰–槐糖苷–5–丙二酰–葡萄糖苷,分别达到总量的46.51%、56.04%、46.38%和68.96%。紫结球甘蓝主要花青素苷成分为矢车菊–3–芥子酰–槐糖苷–5–葡萄糖苷,矢车菊–3–槐糖苷–5–葡萄糖苷和矢车菊–3–p–香豆酰–槐糖苷–5–葡萄糖苷,分别达到总量的41.87%、20.58%和16.02%。花青素苷含量最高的是红叶芥菜,为719.04μg·g-1 FW;其次是紫红色大白菜,为604.03μg·g-1 FW;第三是紫结球甘蓝,为264.96μg·g-1 FW;最低的是红菜薹和紫色白菜,分别为219.07和130.02μg·g-1 FW。紫红色大白菜中检测到2种特有的矢车菊素苷和1种矮牵牛素苷。
Anthocyanins cause red or purple color in Brassica vegetables. The HPLC-MS method was used to analyze components and amounts of anthocyanins in purple heading Chinese cabbage(Brassica rapa L. ssp. pekinensis),purple pakchoi and caixin(Brassica rapa L. ssp. pekinensis),red cabbage(Brassica oleracea L. var. capitata),and red leaf mustard(Brassica juncea Coss.). Totally 20 anthocyanins were separated and identified in these Brassicas. They were major cyaniding,minor delphinidin and petunidin. The main anthocyanins in red leaf mustard,purple heading Chinese,pakchoi and caixin were acylated cyanidin 3-sophoroside-5-glucosides,acylated cyanidin 3,5-diglucosides and cyaniding-3-sinapoylferuloylsophoroside-5-malonylglucoside,accounting for 46.51%,56.04%,46.38% and 68.96% of total anthocyanins. The main anthocyanins in red cabbage were cyanidin 3-sinapoylsophoroside-5-glucoside,cyanidin 3-sophoroside-5-glucoside,cyanidin 3-p-coumaroylsophoroside-5-glucoside,accounting for 41.87%,20.58% and 16.02% of total anthocyanins. The total amounts of anthocyanins were 719.04 μg · g-1 FW in red leaf mustard,604.03 μg · g-1 FW in purple heading Chinese cabbage,264.96 μg · g-1 FW in red cabbage,219.07 μg · g-1 FW in purple caixin and 130.02 μg · g-1 FW in purple pakchoi,respectively. Two specific acylated cyanidin 3-sophoroside-5-glucosides and one specific petundin-3,5-diglucoside were identified in purple heading Chinese cabbage.
出处
《园艺学报》
CAS
CSCD
北大核心
2014年第7期1451-1460,共10页
Acta Horticulturae Sinica
基金
国家‘973’计划项目(2012CB113900)
‘十二五’农村领域国家科技计划项目(2012AA100100)
国家现代农业产业技术体系建设专项资金项目(CARS-25-A-01)
农业部园艺作物生物学与种质创制重点实验室项目
