摘要
白及为兰科植物白及(Bletilla striata(Thunb.)Reichb.f.)的干燥块茎,是一种重要的中药材,近年来种植面积不断扩大。白及花一般呈紫色,可能富含花青素并具有花青素的生物活性,但未见相关研究报道。本研究采用p H示差法测定发现白及花的花青素含量为0.46 mg/g,高效液相色谱法测定发现其含有飞燕草色素(114.528μg/g)、牵牛花色素(22.737μg/g)、天竺葵色素(23.736μg/g)、芍药色素(9.030μg/g)和锦葵色素(27.831μg/g)。体外测定发现白及花提取液具有抗氧化活性,总抗氧化能力为173.1 U/m L,羟自由基清除活性为147.36 U/m L,DPPH自由基清除率为85.43%和超氧阴离子清除率为18.02%。感官审评发现,白及花经沸水浸泡后,汤色呈紫色、透明,具淡雅花香,滋味稍涩、回甘。以上研究表明,白及花富含花青素,具有抗氧化活性,可作为饮料开发。
Bletilla striata is a dry tuber of Orchidaceae Bletilla striata（Thunb.） Reichb. f. and it is an important Chinese herbal medicine. In recent years, the planting area of Bletilla striata is increasing. The flowers of Bletilla striata are generally purple which may be rich in anthocyanins with the biological activity of anthocyanins.Howerer, there was no relevant research report. In this research, the content of anthocyanins in Bletilla striata’s flowers was 0.46 mg/g by p H-differential method. The high performance liquid chromatography（HPLC） showed that it contained delphindin（114.528 μg/g）, petunidin chloride（22.737 μg/g）, pelargonidin chloride（23.736 μg/g）,paeonidin（9.030 μg/g） and malvidin（27.831 μg/g）. In vitro assay found that the extract of Bletilla striata’s flowers had antioxidant activity. The total antioxidant capacity was 173.1 U/m L, the hydroxyl radical scavenging activity was 147.36 U/m L, the DPPH free radical scavenging rate was 85.43% and the superoxide anion scavenging rate was 18.02%. Sensory evaluation showed that the soup color was purple and transparent of Bletilla striata’s flowers by soaking in boiling water. Besides, it tasted slightly astringent and sweet after taste with elegant fragrance. The above studies indicated that Bletilla striata’s flowers were rich in anthocyanins, with the antioxidant activity and could be developed as beverage.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2017年第12期5269-5276,共8页
Genomics and Applied Biology
基金
云南省中青年学术技术带头人后备人才培养项目(2014HB011)资助
关键词
白及花
花青素
抗氧化活性
Bletilla striata flowers
Anthocyanins
Antioxidant activity
