摘要
采用免疫组织化学Ｓ－Ｐ法，对成年（２～３月龄）和老年（２０～３６月龄）Ｗｉｓｔａｒ大鼠耳蜗核组织进行ＣｈＡＴ免疫组织化学显色，并利用计算机图像分析系统对免疫反应阳性细胞进行计数。观察老年大鼠耳蜗核组织胆碱乙酰化酶（ＣｈＡＴ）免疫反应的变化，研究其与老年性聋的发病关系。结果发现ＣｈＡＴ免疫反应阳性神经元主要散在分布于背侧核（ＤＣＮ）的梭型细胞层，老年大鼠耳蜗背侧核ＣｈＡＴ阳性细胞数目少于成年组（Ｐ＜０．００１）。实验结果提示，耳蜗核ＣｈＡＴ阳性细胞数目的减少可能与老年性聋的发病有关。
The purpose of the experiment is to observe the change of choline acetyltransferase (ChAT) immunoreactivity in aged rat cochlear nucleus in order to study its relation with the initiation of presbycusis. The cochlear nucleuses of adult (2～3months) and aged (20～36months) Wistar rats were stained with streptavidin/peroxidase (S P) technique. ChAT immunopositive neurons were counted with a computer aid image analysis system. The result showed that scattered ChAT immunopositive cell bodies were mainly found in the fusiform cell layer of the dorsal cochlear nucleus (DCN). The number of ChAT immunopositive neurons decreased significantly is DCN of aged rat cochlear nucleus ( P <0 001). This indicates that the reduction of ChAT positive neurons in aged rat cochlear nucleus may be related to presbycusis.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
1998年第4期113-116,共4页
Chinese Journal of Histochemistry and Cytochemistry
基金
国家自然科学基金
关键词
耳蜗核
胆碱乙酰化酶
免疫组织化学
大鼠
Cochlear nucleus
Choline acetyltransferase
Immunohistochemistry
Rat
