摘要
目的探讨老年性聋大鼠听皮层病理变化。方法通过甲苯胺蓝染色和免疫组化技术检测并比较正常成年大鼠(对照组,20只)、正常听力老年大鼠(18月龄)(老年组,20只)及以D-半乳糖建造的老年性聋大鼠(实验组,20只)听皮层神经元的形态、数量和兴奋性神经递质乙酰胆碱(Ach)、谷氨酸(Glu)及抑制神经递质γ-氨基丁酸(GABA)的阳性表达数量。结果实验组大鼠较老年组和对照组大鼠听皮层神经元数量减少(P<0.05),而老年组大鼠与对照组差异无统计学意义(P>0.05)。实验组大鼠较老年组和对照组的ACh、GABA表达下调(P<0.01),Glu的表达上调(P<0.01)。结论听皮层神经元的减少、神经递质Ach、GABA表达下调和Glu表达上调为老年性聋听皮层病理表现,可能与老年性聋的发病有关。
Objective To study the pathological changes in auditory cortex of aged rat. Methods Toluidine blue staining and immunohistochemistry were used to detect and compare the morphous and number of neurons and the content of two kinds of excitatory neurotransmitter - acetylcholine （Ach） and glutamic acid （Glu） as well as one inhibitory neurotransmitter - γ--aminobutyric acid （GABA） in auditory cortex in normal adult rats, aged rats with normal hearing （18 months old） and model rats of presbycusis established by D--galactose. Results 1. Comparing normal adult rats and aged rats with normal hearing, the number of neurons in auditory cortex of model rats of presbycusis were obviously reduced （P〈0.05）while there were no visible changes between normal adult rats and aged rats with normal hearing（P〉0.05）. Comparing normal adult rats and aged rats with normal hearing, the content of Aeh and GABA in model rats of presbycusis were significantly increased （P〈0.01） while Glu significantly decreased（P〈0.01）. Conclusion Presbycusis may be related to reduced neurons number, decreased Ach and GABA content and increased Glu content in auditory cortex.
出处
《听力学及言语疾病杂志》
CAS
CSCD
北大核心
2011年第1期47-51,共5页
Journal of Audiology and Speech Pathology
基金
福建省科技计划重点项目(2009Y0041)
关键词
老年性聋
听皮层
神经元
神经递质
Presbycusis
Auditory cortex
Nerve cell
Neurotransmitter
