摘要
文章利用巴氏芽孢杆菌的微生物矿化作用产生的碳酸钙沉积,对透明人工粗糙单裂隙中残留的甲苯团块进行了封堵试验,对裂隙两端水头、出水口甲苯浓度、DO值及裂隙内部状况进行了监测。试验结果表明,该生物封堵技术能在较短时间内在甲苯团块四周形成碳酸钙封闭环,8d时间内将单裂隙出水口甲苯浓度降低90%以上。同时,裂隙等效水力隙宽降低约60%,表明碳酸钙沉积更多的是发生在甲苯团块四周。切断营养物质供应后的一段时间内,碳酸钙封闭环仍然能保持作用,阻隔甲苯与水流之间的水力联系。在不施加任何营养物质的条件下,巴氏芽孢杆菌能将甲苯作为其新陈代谢的碳源,对甲苯具有一定的降解作用。DO监测数据表明,巴氏芽孢杆菌在对甲苯团块封堵过程中,氧气的消耗十分有限,表明该技术可应用于地下缺氧环境。
The blocking of residual toluene agglomerates in transparent artificial rough single cracks was carried out by calcium carbonate deposition by microbial mineralization of Sporosarcina pasteurii.Toluene concentration,DO value and internal condition of cracks at the water head and outlet of both ends of the fracture were monitored.The results show that the bio-blocking technology can form a calcium carbonate closed ring around the toluene mass in a short time,and reduce the toluene concentration of the single-fracture outlet by more than 90%in 8 days.At the same time,the fracture equivalent hydraulic gap is reduced by about 60%,indicating that calcium carbonate deposition occurs more around the toluene mass.The calcium carbonate closed loop remains in place for a period of time after the supply of nutrients is cut off,blocking the hydraulic connection between toluene and water flow.Sporosarcina pasteurii can use toluene as a carbon source for its metabolism without any nutrients,and it has a certain degradation effect on toluene.The DO monitoring data showed that the oxygen consumption of Bacillus bacillus in the process of blocking the toluene mass was very limited,indicating that the technology can be applied to the underground anoxic environment.
作者
高杰
谈叶飞
沙海飞
陈舟
GAO Jie;TAN Yefei;SHA Haifei;CHEN Zhou(Shaanxi Provincial Water Resources and Reservoir Dispatch Management Center,Xi'an 718900,China;Nanjing Hydraulic Research Institute,Nanjing 210029,China;Hohai University,Nanjing 210024,China)
出处
《环境科学与技术》
CAS
CSCD
北大核心
2019年第11期75-79,共5页
Environmental Science & Technology
基金
国家重点研发计划(2018YFC1508604)
南京水利科学研究院院基金(Y118015)
交通运输行业重点科技项目(2018-ZD3-025).
