摘要
用鸡胚脑和兔脑神经细胞研究了氟丙烯（ＡＣ）作用下的细胞内游离钙浓度（［ｃａ２＋］ｉ），细胞骨架形态，细胞骨架蛋白的合成及单位面积离体管蛋白聚合数量的改变。结果显示，在ＡＣ的作用下，［Ｃａ２＋］ｉ随着毒物浓度增高而明显增加；光镜下细胞骨架形态也随ＡＣ增加而由网状分布到断裂，破碎，最后仅剩下细胞核；细胞骨架蛋白的合成随毒物浓度的增高而明显抑制；Ｃａ２＋直接抑制离体管蛋白聚合；随着ＡＣ的浓度增高，单位面积内管蛋白聚合的数量也下降。研究提示ＡＣ引起的周围神经病可能与细胞内Ｃａ２＋稳态失衡及细胞骨架受损有关．
Using nerve cells from chicken em-bryo and rabbit brain, the changes of intracellular freecalcium([ Ca2+]i),cytoskeleton morphology,cytoske-leton protein synthesis and tubulin polymerization in-duced by allyl chloride were studied,The results hadshown that after the addition of allyl chloride,[Ca2+]i was significantly increased,cytoskeleton mor-phology was obviously changed from normal to disor-der and breakage,cytoskeleton protein synthesis wasmarkedly inhibited.Ca2+ directly inhibited tubulinpolymerization in vitro and the amount of tubulinpolymerization into microtubules fell down. The studyrevealed that allyl chloride could induce unbalancedintracellular calcium homeostasis,cytoskeleton injury,and toxic peripheral neuropathy as well.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
1995年第4期292-295,共4页
Chinese Journal of Pharmacology and Toxicology
基金
国家自然科学基金
关键词
氯丙烯
钙
微管蛋白
细胞骨架
神经毒理学
allyl chloride
calcium
tubulin
cytoskeleton
neurotoxicology 1The project was supported by the National Nature Sci-ence Foundation of China, No 39170661
