摘要
目的探讨腺苷酸环化酶(AC)在氯丙烯引起的中毒性周围神经病发病过程中的作用。方法Wistar雄性大鼠ig氯丙烯100或200mg·kg-1,每周3次,连续3个月。Western印迹法分析测定大鼠大脑、小脑、脊髓和坐骨神经中AC蛋白含量。结果在大脑、小脑、脊髓和坐骨神经上清中,氯丙烯100mg·kg-1组AC蛋白含量分别降低43%,43%,32%和31%,200mg·kg-1组分别降低51%,74%,39%和70%,与对照组相比均有显著性差异。结论氯丙烯使神经组织中AC含量明显降低,提示AC含量降低可能是氯丙烯导致中毒性周围神经病的发病机制之一。
AIM To clarify the role of adenylyl cyclase（AC） in allyl chloride-induced toxic peripheral neuropathies. METHODS Wistar male rats were treated by allyl chloride 100 and 200 mg·kg^-1, ig, 3 times a week for continuous 3 months. AC contents in nerve tissues were analyzed by Western blotting. RESULTS suspension of cerebrum, cerebellum, spinal cord and sciatic nerve, AC contents decreased by 43%, 43%, 32% and 31%, respectively, in 100 mg·kg^-1 group, and by 51%, 74%, 39% and 70%, respectively, in 200 mg·kg^-1 group.CONCLUSION Allyl chloride can decrease the contents of AC in nerve tissues of rats, which may be one of the toxic mechanism of allyl chlorideinduced toxic peripheral neuropathies.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
2005年第4期308-310,共3页
Chinese Journal of Pharmacology and Toxicology
基金
国家重点基础研究发展计划项目(2002CB512907)
国家自然科学基金项目(30271138)~~
关键词
氯丙烯
腺苷酸环化酶
神经组织
allyl chloride
adenylate cyclase
nerve tissue
