Purpose: To investigate changes in the production of tissue inhibitor of metalloproteinase type 1 (TIMP-1) by human retinal pigment epithelial (RPE) cells following argon laser exposure.Methods: Human cultured ARPE19 ...Purpose: To investigate changes in the production of tissue inhibitor of metalloproteinase type 1 (TIMP-1) by human retinal pigment epithelial (RPE) cells following argon laser exposure.Methods: Human cultured ARPE19 cells were exposed to argon green laser at four different energy levels ranging from 60mW to 360mW. After laser exposure, the culture media were sampled at 0, 24, 72 and 144 hours for TIMP-1 concentration produced by the RPE cells. The levels of TIMP-1 in the cells treated with different laser energy levels were compared with a control group not exposed to laser application.Immunocytochemistry for proliferating cell nuclear antigen (PCNA) was performed to detect any adverse effects on the RPE cells caused by laser exposure.Results: Immediately after laser exposure, the concentration of TIMP- 1 was not detectable. At 24 hours after laser exposure, the concentration of TIMP-1 increased significantly in RPE cells treated with 120mW and 240mW at 24 hours (P=0.006 and P=0.001respectively) compared with control cells. At 72 hours after treatment, RPE cells treated at 120mW, 240mW and 360mW demonstrated significantly increase in TIMP-1production compared with control (P=0.003, P ＜ 0.001 and P ＜ 0.001, respectively).No significant reduction in cell viability was observed following laser application as detected by PCNA expression.Conclusions: Our results demonstrated that early TIMP-1 production by RPE cells in cell cultures was enhanced following laser exposure.展开更多
目的研究骨巨细胞瘤组织中基质金属蛋白酶-2(Matrix metalloproteinase-2)及其特异性抑制剂金属蛋白酶组织抑制因子-2(Tissue inhibitor of metalloproteinase-2)的表达与肿瘤血管形成和关系。方法用免疫组化SP法检测45例骨巨细胞瘤组织...目的研究骨巨细胞瘤组织中基质金属蛋白酶-2(Matrix metalloproteinase-2)及其特异性抑制剂金属蛋白酶组织抑制因子-2(Tissue inhibitor of metalloproteinase-2)的表达与肿瘤血管形成和关系。方法用免疫组化SP法检测45例骨巨细胞瘤组织中MMP-2、TIMP-2的表达情况,以CD31标记检测微血管密度(MVD),计算肿瘤复发组和非复发组MMP-2/TIMP-2的比值,分析这些指标与骨巨细胞瘤预后的关系。结果骨巨细胞瘤组织有高水平的MMP-2、TIMP-2和CD31表达。复发组表达MMP-2、MMP-2/TIMP-2比值以及MVD均显著高于非复发组,但与组织学分级无关。MMP-2与MVD呈正的直线相关。结论MMP-2/TIMP-2比值升高与骨巨细胞瘤的复发以及肿瘤血管形成有关。展开更多
文摘急性肾损伤(acute kidney injury,AKI)是一种常见的术后并发症,尚无确切的生物标志物。金属蛋白酶2组织抑制剂(tissue inhibitor of metalloproteinases-2,TIMP-2)和胰岛素样生长因子结合蛋7(insulin-like growth factor-binding protein 7,IGFBP7)属于细胞周期阻滞生物标志物。近年众多临床研究发现尿液中TIMP-2联合IGFBP7的含量可较好地预测中重度AKI。2014年美国食品和药物管理局批准上市了"NephroCheck?"(尿[TIMP-2]×[IGFBP7]的检测)。本文将综述尿液中细胞周期阻滞生物标志物[TIMP-2]×[IGFBP7]的含量对手术后AKI风险预测的研究。
文摘Purpose: To investigate changes in the production of tissue inhibitor of metalloproteinase type 1 (TIMP-1) by human retinal pigment epithelial (RPE) cells following argon laser exposure.Methods: Human cultured ARPE19 cells were exposed to argon green laser at four different energy levels ranging from 60mW to 360mW. After laser exposure, the culture media were sampled at 0, 24, 72 and 144 hours for TIMP-1 concentration produced by the RPE cells. The levels of TIMP-1 in the cells treated with different laser energy levels were compared with a control group not exposed to laser application.Immunocytochemistry for proliferating cell nuclear antigen (PCNA) was performed to detect any adverse effects on the RPE cells caused by laser exposure.Results: Immediately after laser exposure, the concentration of TIMP- 1 was not detectable. At 24 hours after laser exposure, the concentration of TIMP-1 increased significantly in RPE cells treated with 120mW and 240mW at 24 hours (P=0.006 and P=0.001respectively) compared with control cells. At 72 hours after treatment, RPE cells treated at 120mW, 240mW and 360mW demonstrated significantly increase in TIMP-1production compared with control (P=0.003, P ＜ 0.001 and P ＜ 0.001, respectively).No significant reduction in cell viability was observed following laser application as detected by PCNA expression.Conclusions: Our results demonstrated that early TIMP-1 production by RPE cells in cell cultures was enhanced following laser exposure.
文摘目的研究骨巨细胞瘤组织中基质金属蛋白酶-2(Matrix metalloproteinase-2)及其特异性抑制剂金属蛋白酶组织抑制因子-2(Tissue inhibitor of metalloproteinase-2)的表达与肿瘤血管形成和关系。方法用免疫组化SP法检测45例骨巨细胞瘤组织中MMP-2、TIMP-2的表达情况,以CD31标记检测微血管密度(MVD),计算肿瘤复发组和非复发组MMP-2/TIMP-2的比值,分析这些指标与骨巨细胞瘤预后的关系。结果骨巨细胞瘤组织有高水平的MMP-2、TIMP-2和CD31表达。复发组表达MMP-2、MMP-2/TIMP-2比值以及MVD均显著高于非复发组,但与组织学分级无关。MMP-2与MVD呈正的直线相关。结论MMP-2/TIMP-2比值升高与骨巨细胞瘤的复发以及肿瘤血管形成有关。
