Background Autologous transplantation of the submandibular gland （SMG） into the temporal fossa with microvascular anastomosis has been successfully applied in severe xerophthalmia patients as a permanent tear substi...Background Autologous transplantation of the submandibular gland （SMG） into the temporal fossa with microvascular anastomosis has been successfully applied in severe xerophthalmia patients as a permanent tear substitute. However, severe xerophthalmia can be accompanied by salivary gland dysfunction, making such autotransplantation unsuitable. Therefore, SMG allotransplantation might be a solution. The aim of this study was to assess the technical feasibility of submandibular gland allotransplantation. Methods Twelve miniature swine were randomized to serve as donors or recipients. One SMG was transplanted between a donor and a recipient. The donor SMG was revascularized by microvascular anastomosis of its vascular pedicle to the recipient lingual artery and external jugular vein. The secretory duct was implanted into the vestibule of the mouth through a subcutaneous tunnel. No ilnmunosuppressive agent was administered. The results were assessed by visual inspection of the secretion, and histopathological examination of the transplanted SMG. Results Technically, all surgical procedures were successful. Clear secretion flowed out of the duct as soon as blood supply of the transplanted submandibular gland was reestablished. The secretion of the gland lasted for 5 days. As expected, an acute rejection reaction occurred after surgery because no immunosuppressive agents were used. Secretion from the transplanted SMG ceased within 5 days. Conclusions A model of SMG allotransplantation can be established in miniature swine. The technique of submandibular gland allotransplantation is feasible.展开更多
文摘目的:探索应用高脂饲料加球囊拉伤的方法制备小型猪慢性心肌缺血模型。方法:先给予小型猪高脂饲料喂养2周后,戊巴比妥钠麻醉下分离右侧颈总动脉,以Launcher 6F右冠导引导管置于左冠状动脉开口,造影后置入球囊导丝,在导丝指引下置入2.5mm或3.0mm球囊至冠状动脉左前降支(left anterior descending,LAD)中部,以10atm打开球囊堵闭LAD,扩张3次。术后继续给予高脂饲料2月。结果:给予高脂饲料和LAD球囊扩张拉伤2月后,小型猪LAD造影显示内径变细,内壁粗糙,血清甘油三酯(triglycerides,TRIG)、胆固醇(cholesterol,CHO)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)、CHO/HDL-C比值均明显升高,猪LAD血管内膜增生变厚,内部可见丰富的泡沫细胞,并有粥样硬化改变,部分变性、坏死的心肌出现心肌纤维化增生。结论:应用高脂饲料加球囊拉伤的方法制备的小型猪模型,初步表现出慢性心肌缺血的病理改变。
基金This study was supported by grants from the National Nature Science Foundation of China (No. 30340066)the Ministry of Science and Technology (No. 2003ccc00800).
文摘Background Autologous transplantation of the submandibular gland （SMG） into the temporal fossa with microvascular anastomosis has been successfully applied in severe xerophthalmia patients as a permanent tear substitute. However, severe xerophthalmia can be accompanied by salivary gland dysfunction, making such autotransplantation unsuitable. Therefore, SMG allotransplantation might be a solution. The aim of this study was to assess the technical feasibility of submandibular gland allotransplantation. Methods Twelve miniature swine were randomized to serve as donors or recipients. One SMG was transplanted between a donor and a recipient. The donor SMG was revascularized by microvascular anastomosis of its vascular pedicle to the recipient lingual artery and external jugular vein. The secretory duct was implanted into the vestibule of the mouth through a subcutaneous tunnel. No ilnmunosuppressive agent was administered. The results were assessed by visual inspection of the secretion, and histopathological examination of the transplanted SMG. Results Technically, all surgical procedures were successful. Clear secretion flowed out of the duct as soon as blood supply of the transplanted submandibular gland was reestablished. The secretion of the gland lasted for 5 days. As expected, an acute rejection reaction occurred after surgery because no immunosuppressive agents were used. Secretion from the transplanted SMG ceased within 5 days. Conclusions A model of SMG allotransplantation can be established in miniature swine. The technique of submandibular gland allotransplantation is feasible.
