摘要
目的 研究重组纳豆激酶（recombinant nattokinase,r-NK）对中国实验小型猪冠状动脉电刺激血栓的溶栓作用和对心肌缺血再灌注损伤的保护作用.方法 采用直接电刺激损伤中国实验小型猪冠状动脉内膜造成冠脉内血栓.运用冠状动脉血管造影和多媒体图像分析判断冠脉的再通情况;用心外膜电图描记和硝基四氮唑蓝（N-BT）方法计算心肌缺血程度、范围及梗死面积;同时测定肌酸磷酸激酶-MB型（CK-MB）的活性.结果 r-NK 0.25～0.5 mg·kg^-1能促使冠脉血栓溶解,显著缩小血栓面积,促进血管的再通;同时可明显减轻心肌缺血程度（∑-ST）,缩小心肌梗死面积,降低心肌梗死动物血清中CK-MB活性.结论 r-NK能抑制电刺激冠脉血栓的形成,促使血栓的溶解,并能减轻血管再通后缺血再灌注引起的心肌损伤.
OBJECTIVE To study the thrombolytic effects of r-NK on coronary thrombosis induced by electrical stimulation, and the protective effects on myocardial damage subjected to ischemia reperfusion in Chinese experimental miniature swine. METHODS Endoarterium was injuried and coronary thrombi were formed gradually through direct electrical stimulation on the coronary artery of animals. Artery rechannelization was analyzed by coronary artery angiograph and multiple media graphic analysis. The experiments adopted epicardiogram mapping to measure the scope and degree of myoeardiul ischemia, quantitafive histologic assay （nitroblue tetrazolium, N-BT stain） to determine the size of myocardial infarction. Serum creatine phosphokinase-MB （CK-MB） activity was detected. RESULIS R-NK 0.25 - 0.5 mg· kg^-1 improved coronary thrombolysis, lessened the thrombi area, and affiliated artery rechannelization. Furthermore, r-NK alleviated the degree of myocardial ischemia （∑-ST）, narrowed the ischemic area and inhibited the CK-MB activity. CONCLUSION R-NK can inhibit coronary thrombosis in- duced by electrical stimulation, improve thrombolysis, and alleviate myocardial damage subjected to ischemia reperfusion after artery rechannelizaiton.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2006年第8期589-592,共4页
Chinese Pharmaceutical Journal
