摘要
目的研究重组纳豆激酶(recombinant nattokinase,r-NK)大剂量重复给药对Beagle犬和小型猪凝血和纤溶系统的不同影响。方法用血球计数仪测定血小板相关参数;用血凝仪采取凝固法依次测定纤维蛋白原(Fib)含量、活化部分凝血酶时间(APTT)、凝血酶原时间(PT)、凝血酶时间 (TT);以ELISA法分别测定纤溶酶原(PLG)、纤溶酶原激活因子(t-PA)活性。结果药物对Beagle 犬和小型猪有相似的血管刺激作用。给药1周后Beagle犬血小板计数下降,PT明显延长,Fib含量明显降低,PLG活性升高。而药物对小型猪的各项参数均无明显影响。结论不同动物对相同剂量r-NK毒性反应存在较大的差异性,r-NK大剂量重复给药可破坏Beagle犬的凝血/纤溶系统平衡,而不影响小型猪凝血/纤溶系统功能。
Objective To study the different effects of recombinant nattokinase （r-NK） on coagulation and fibrinolysis system in Beagle dogs and in miniature swine when treated repeatedly at large dosage. Methods Platelet-related parameters were obtained by blood analyzer. The fibrinogen （Fib）, activated partial thrombin time （APTF）, prothrombin time （PT） and thrombin tim, （TT） were determined by coagulation method with the coagulation detector. Plasmmogen （PLG） and tissue-type ptasminogen activator （t-PA） were tested by ELISA. Results Repeated administration presented the same blood vessel irritation both in Beagle dogs and miniature swine. After 1 week of administration, the number of platelets was decreased, PT was prolonged, Fib concentration decreased and the activity of PLG were increased in Beagle dogs. However, r-NK had no effects＇ on the above parameters in miniature swine. Conclusion There was toxic difference between different animals towards r-NK at the same dosage. In Beagle dogs, r-NK could break the balance between the coagulation and fibrinolysis system. Whereas r- NK had no effects on the coagulation and fibrinolysis system in miniature swine.
出处
《实验动物与比较医学》
CAS
2006年第1期16-18,共3页
Laboratory Animal and Comparative Medicine
基金
国家"863"计划重大专项基金资助课题(No.2002AA2Z346B)。
