It is attractive and encouraging to develop new fluorescent carbon dots(CDs)with excellent optical properties and promising applications prospects.Herein,highly-efficient green emissive CDs(m-CDs)with a high quantum y...It is attractive and encouraging to develop new fluorescent carbon dots(CDs)with excellent optical properties and promising applications prospects.Herein,highly-efficient green emissive CDs(m-CDs)with a high quantum yield(QY)of 71.7%in water are prepared through a facile solvothermal method.Interestingly,the m-CDs exhibit excellent fluorescence stability in the pH range of 1–9.However,the fluorescence intensity of the m-CDs is almost completely quenched as the pH is increased from 9 to 10.The mechanism of the unique pH-responsive behavior is discussed in detail and a plausible mechanism is proposed.Owing to the unique pH-responsive behavior,the m-CDs are used as a on-off fluorescent probe for water quality identification.By combining the reversible pH-ultrasensitive optical properties of the m-CDs in the pH range of 9–10 with the glucose oxidase-mimicking(GOx-mimicking)ability of Au nanoparticles(AuNPs),glucose can be quantitatively detected.Finally,two environment-friendly starch-based solid-state fluorescence materials(powder and film)are developed through green preparation routes.展开更多
A novel ultrasensitive detection in a small volume of a low refractive index liquid via Surface enhanced Raman scattering(SERS)-active liquid core waveguide is described. The liquid core waveguide demonstrated was con...A novel ultrasensitive detection in a small volume of a low refractive index liquid via Surface enhanced Raman scattering(SERS)-active liquid core waveguide is described. The liquid core waveguide demonstrated was constructed by using the light-guiding silica capillary modified with SERS-active nanoparticles on the inner surface of the fiber wall. The Raman signal of the sample can be remarkably enhanced by the immobilized Ag nanoparticles. The laser and SERS scattering from the flowing liquid in the core possess the totally reflective waveguide model within the fiber wall. Thus, the sample dissolved in the low refractive index liquid, e.g. methanol, can be quantitatively monitored by Raman spectra in the liquid core cell and the detection limit can reach 10 -8 mol/L.展开更多
Aptamer serves as a potential candidate for the micro-detection of cocaine due to its high specificity,high affinity and good stability.Although cocaine aptasensors have been extensively studied,the binding mechanism ...Aptamer serves as a potential candidate for the micro-detection of cocaine due to its high specificity,high affinity and good stability.Although cocaine aptasensors have been extensively studied,the binding mechanism of cocaine-aptamer interactions is still unknown,which limits the structural refinement in the design of an aptamer to improve the performance of cocaine aptasensors.Herein,we report a label-free,ultrasensitive detection of single-molecule cocaine-aptamer interaction by using an electrical nanocircuit based on graphene-moleculegraphene single-molecule junctions (GMG-SMJs).Real-time recordings of cocaine-aptamer interactions have exhibited distinct current oscillations before and after cocaine treatment,revealing the dynamic mechanism of the conformational changes of aptamer upon binding with cocaine.Further concentration-dependent experiments have proved that these devices can act as a single-molecule biosensor with at least a limit of detection as low as 1 nmol·L^-1.The method demonstrated in this work provides a novel strategy for shedding light on the interaction mechanism of biomolecules as well as constructing new types of aptasensors toward practical applications.展开更多
Gastric cancer(GC)is a prevalent malignant tumor within the digestive system,with over 40%of new cases and deaths related to GC globally occurring in China.Despite advancements in treatment modalities,such as surgery ...Gastric cancer(GC)is a prevalent malignant tumor within the digestive system,with over 40%of new cases and deaths related to GC globally occurring in China.Despite advancements in treatment modalities,such as surgery supplemented by adjuvant radiotherapy or chemotherapeutic agents,the prognosis for GC remains poor.New targeted therapies and immunotherapies are currently under invest-igation,but no significant breakthroughs have been achieved.Studies have indicated that GC is a heterogeneous disease,encompassing multiple subtypes with distinct biological characteristics and roles.Consequently,personalized treatment based on clinical features,pathologic typing,and molecular typing is crucial for the diagnosis and management of precancerous lesions of gastric cancer(PLGC).Current research has categorized GC into four subtypes:Epstein-Barr virus-positive,microsatellite instability,genome stability,and chromosome instability(CIN).Technologies such as multi-omics analysis and gene sequencing are being employed to identify more suitable novel testing methods in these areas.Among these,ultrasensitive chromosomal aneuploidy detection(UCAD)can detect CIN at a genome-wide level in subjects using low-depth whole genome sequencing technology,in conjunction with bioinformatics analysis,to achieve qualitative and quantitative detection of chromosomal stability.This editorial reviews recent research advancements in UCAD technology for the diagnosis and management of PLGC.展开更多
In this manuscript,we first report an ultrasensitive detection assay of microRNA by combing asymmetric polymerase chain reaction(A-PCR)and loop-mediated isothermal amplification(LAMP)technology.Using A-PCR obtained an...In this manuscript,we first report an ultrasensitive detection assay of microRNA by combing asymmetric polymerase chain reaction(A-PCR)and loop-mediated isothermal amplification(LAMP)technology.Using A-PCR obtained an extended single strand to form LAMP stem-loop structure under isothermal amplification conditions.We used miRNAs as a loop primer probe in LAMP reaction and completed its ultrasensitive and rapid detection.The established method furnished a fast,specific and efficient detection of target miRNA with a detection limit as low as 10 amol/L in 90 min.展开更多
Two-dimensional covalent organic framework(COF)has distinctive properties that offer potential opportunities for developing advanced electrode materials.In this work,a core-shell material composed of TAPB-DMTP-COF(TAP...Two-dimensional covalent organic framework(COF)has distinctive properties that offer potential opportunities for developing advanced electrode materials.In this work,a core-shell material composed of TAPB-DMTP-COF(TAPB,1,3,5-tris(4-aminophenyl)benzene;DMTP,2,5-dimethoxyterephaldehyde)core and conducting polymer shell,TAPB-DMTP-COF@PANI,was synthesized solvothermally using a polymerization method.The structural cha racteristics of the prepared composite were revealed by X-ray diffraction patterns(XRD),fourier transform infrared spectra(FTIR),X-ray photoelectron spectroscopy(XPS),transmission electron microscopy(TEM).The electrochemical analyses were verified by subsequent monitoring of trace levels of acetaminophen.This resultant composite not only facilitated acetaminophen to interact with absorption sites byπ-πstacking effect and hydrogen bonding but also overcame the poor conductivity of COF.Under the optimal conditions,a low limit of detection of 0.032μmol/L and wide linear range of 0.10-500μmol/L were obtained.The electrochemical platform was almost unaffected by other interfering substances,and successfully applied for the practical detection of acetaminophen in commercial tablet,human blood serum and urine.The enhanced performance makes this COF based core-shell composite a promising material in electrochemical senso r.展开更多
基金supported by the National Natural Science Foundation of China(Nos.21375123,21675151,and 21721003)the Ministry of Science and Technology of China(No.2016YFA0203203).
文摘It is attractive and encouraging to develop new fluorescent carbon dots(CDs)with excellent optical properties and promising applications prospects.Herein,highly-efficient green emissive CDs(m-CDs)with a high quantum yield(QY)of 71.7%in water are prepared through a facile solvothermal method.Interestingly,the m-CDs exhibit excellent fluorescence stability in the pH range of 1–9.However,the fluorescence intensity of the m-CDs is almost completely quenched as the pH is increased from 9 to 10.The mechanism of the unique pH-responsive behavior is discussed in detail and a plausible mechanism is proposed.Owing to the unique pH-responsive behavior,the m-CDs are used as a on-off fluorescent probe for water quality identification.By combining the reversible pH-ultrasensitive optical properties of the m-CDs in the pH range of 9–10 with the glucose oxidase-mimicking(GOx-mimicking)ability of Au nanoparticles(AuNPs),glucose can be quantitatively detected.Finally,two environment-friendly starch-based solid-state fluorescence materials(powder and film)are developed through green preparation routes.
文摘A novel ultrasensitive detection in a small volume of a low refractive index liquid via Surface enhanced Raman scattering(SERS)-active liquid core waveguide is described. The liquid core waveguide demonstrated was constructed by using the light-guiding silica capillary modified with SERS-active nanoparticles on the inner surface of the fiber wall. The Raman signal of the sample can be remarkably enhanced by the immobilized Ag nanoparticles. The laser and SERS scattering from the flowing liquid in the core possess the totally reflective waveguide model within the fiber wall. Thus, the sample dissolved in the low refractive index liquid, e.g. methanol, can be quantitatively monitored by Raman spectra in the liquid core cell and the detection limit can reach 10 -8 mol/L.
基金The authors ack no wledge primary fin ancial support from the National Key R&D Program of China (No. 2017YFA0204901)the National Natural Science Foundation of China (No. 21727806)the Natural Science Foundation of Beijing (No. Z181100004418003) and the Interdisciplinary Medicine Seed Fund of Peking University.
文摘Aptamer serves as a potential candidate for the micro-detection of cocaine due to its high specificity,high affinity and good stability.Although cocaine aptasensors have been extensively studied,the binding mechanism of cocaine-aptamer interactions is still unknown,which limits the structural refinement in the design of an aptamer to improve the performance of cocaine aptasensors.Herein,we report a label-free,ultrasensitive detection of single-molecule cocaine-aptamer interaction by using an electrical nanocircuit based on graphene-moleculegraphene single-molecule junctions (GMG-SMJs).Real-time recordings of cocaine-aptamer interactions have exhibited distinct current oscillations before and after cocaine treatment,revealing the dynamic mechanism of the conformational changes of aptamer upon binding with cocaine.Further concentration-dependent experiments have proved that these devices can act as a single-molecule biosensor with at least a limit of detection as low as 1 nmol·L^-1.The method demonstrated in this work provides a novel strategy for shedding light on the interaction mechanism of biomolecules as well as constructing new types of aptasensors toward practical applications.
文摘Gastric cancer(GC)is a prevalent malignant tumor within the digestive system,with over 40%of new cases and deaths related to GC globally occurring in China.Despite advancements in treatment modalities,such as surgery supplemented by adjuvant radiotherapy or chemotherapeutic agents,the prognosis for GC remains poor.New targeted therapies and immunotherapies are currently under invest-igation,but no significant breakthroughs have been achieved.Studies have indicated that GC is a heterogeneous disease,encompassing multiple subtypes with distinct biological characteristics and roles.Consequently,personalized treatment based on clinical features,pathologic typing,and molecular typing is crucial for the diagnosis and management of precancerous lesions of gastric cancer(PLGC).Current research has categorized GC into four subtypes:Epstein-Barr virus-positive,microsatellite instability,genome stability,and chromosome instability(CIN).Technologies such as multi-omics analysis and gene sequencing are being employed to identify more suitable novel testing methods in these areas.Among these,ultrasensitive chromosomal aneuploidy detection(UCAD)can detect CIN at a genome-wide level in subjects using low-depth whole genome sequencing technology,in conjunction with bioinformatics analysis,to achieve qualitative and quantitative detection of chromosomal stability.This editorial reviews recent research advancements in UCAD technology for the diagnosis and management of PLGC.
基金supported by the National Key R&D Program of China (Nos.2017YFA0208100,2016YFA0602900)National Natural Science Foundation of China (Nos.91853124,21778057 and 21420102003)Chinese Academy of Sciences
文摘In this manuscript,we first report an ultrasensitive detection assay of microRNA by combing asymmetric polymerase chain reaction(A-PCR)and loop-mediated isothermal amplification(LAMP)technology.Using A-PCR obtained an extended single strand to form LAMP stem-loop structure under isothermal amplification conditions.We used miRNAs as a loop primer probe in LAMP reaction and completed its ultrasensitive and rapid detection.The established method furnished a fast,specific and efficient detection of target miRNA with a detection limit as low as 10 amol/L in 90 min.
基金supported by the National Natural Science Foundation of China(No.21205103)Jiangsu Provincial Natural Science Foundation(No.BK2012258)+2 种基金Young and Middle-aged Academic Leaders Foundation of Yangzhou UniversityTop-notch Academic Programs Project of Jiangsu Higher Education Institutions(TAPP)funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)。
文摘Two-dimensional covalent organic framework(COF)has distinctive properties that offer potential opportunities for developing advanced electrode materials.In this work,a core-shell material composed of TAPB-DMTP-COF(TAPB,1,3,5-tris(4-aminophenyl)benzene;DMTP,2,5-dimethoxyterephaldehyde)core and conducting polymer shell,TAPB-DMTP-COF@PANI,was synthesized solvothermally using a polymerization method.The structural cha racteristics of the prepared composite were revealed by X-ray diffraction patterns(XRD),fourier transform infrared spectra(FTIR),X-ray photoelectron spectroscopy(XPS),transmission electron microscopy(TEM).The electrochemical analyses were verified by subsequent monitoring of trace levels of acetaminophen.This resultant composite not only facilitated acetaminophen to interact with absorption sites byπ-πstacking effect and hydrogen bonding but also overcame the poor conductivity of COF.Under the optimal conditions,a low limit of detection of 0.032μmol/L and wide linear range of 0.10-500μmol/L were obtained.The electrochemical platform was almost unaffected by other interfering substances,and successfully applied for the practical detection of acetaminophen in commercial tablet,human blood serum and urine.The enhanced performance makes this COF based core-shell composite a promising material in electrochemical senso r.
