摘要
生物标志物的快速、精准检测对控制和预防疾病或病毒的暴发具有重要意义。该研究将三维花状Au@(MoS2/GO/o-MWNTs)纳米复合物(AMGMs)作为一种理想的基底,利用“三明治”免疫夹心组装法构筑一种高灵敏的电化学发光免疫传感器,并用于前列腺特异性抗原(PSA)的高灵敏检测。AMGMs不仅具有良好的导电性和生物相容性,其三维花状的立体结构可提供更多活性位点并加载更多抗体,显著增加了传感器的响应信号。采用电化学交流阻抗法和循环伏安法对组装过程进行跟踪,证实了该传感器组装的可行性,并采用电化学发光法研究了传感器对底液中不同浓度PSA的响应。结果表明,该传感器实现了对肿瘤标志物PSA的灵敏检测,其线性范围为0.1 pg/mL~50 ng/mL,检出限(S/N=3)为0.1 pg/mL,对实际样品的加标回收率为98.2%~106%。该方法的准确度良好,可为临床PSA检测提供准确可靠的新方法。
The accurate and rapid quantitative detection of biomarkers has a significant influence on controlling and preventing disease or toxin outbreaks.In this study,3D flower-like Au@(MoS2/GO/o-MWNTs)nanohybrids(AMGMs)were introduced as a desirable substrate for sensitive electrochemiluminescence(ECL)detection of prostate specific antigen(PSA).Specifically,the AMGMs acted as a substrate material with desirable electrical conductivity,excellent biocompatibility and a large specific surface area for loading abundant primary antibody,which could gather abundant amounts of Ru complexes on the electrode,giving an amplified signal.The assembly process of this immunosensor was investigated by electrochemical impedance spectroscopy(EIS)and cyclic voltammetry(CV),and confirmed by ECL method.The proposed ECL immunosensor was applied in the detection on different contents of PSA in the substrate,with a wide linear range of 0.1 pg/mL-50 ng/mL and a detection limit(S/N=3)of 0.1 pg/mL.The spiked recoveries for actual samples ranged from 98.2%to 106%.The proposed method exhibited the advantages of excellent stability and selectivity in determination of PSA antigen,providing a sensitive and novel ECL analysis platform for the trace detection of other biomarkers.
作者
孙莹莹
吴晓惠
SUN Ying-ying;WU Xiao-hui(Department of Medical Laboratory Science,Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China)
出处
《分析测试学报》
CAS
CSCD
北大核心
2021年第3期363-369,共7页
Journal of Instrumental Analysis
基金
2020年辽宁省百千万人才资助项目(2020921098)
辽宁中医药大学科研启动基金
沈阳市中青年科技创新人才项目(RC180255)。
