No efficient therapy for liver fibrosis has been available. This study wasaimed to provide evidence that the introduction of a plasmid expressing antisense tissue inhibitorof metalloproteinase-1 (TIMP-1) into a rat mo...No efficient therapy for liver fibrosis has been available. This study wasaimed to provide evidence that the introduction of a plasmid expressing antisense tissue inhibitorof metalloproteinase-1 (TIMP-1) into a rat model of immunologically induced liver fibrosis canresult in the increased activity of interstitial collagenase, thus enhancing the degradation ofcollagen. Real-time nested polymerase chain reaction ( RT-Nested-PCR) and gene recombinationtechniques were used to construct a rat antisense TIMP-1 recombinant plasmid that can be expressedin eukaryotic cells. Both the recombinant plasmid and an empty vector ( _(pc)DNA3 ) wereencapsulated with glycosyl-poly-L-lysine and injected into rats suffering from pig serum-inducedliver fibrosis. The expression of ex'ogenous transfected plasmid was assessed by Northern blot,RT-PCR, and Western blot. Hepatic interstitial collagenase activity was detected usingfluorescinisothiocyanate ( FITC)-labeledtype Ⅰ collagen. In addition to hepatic hydroxyprolinecontent, hepatic collagen types Ⅰ and Ⅲ were detected by immunohistochemical staining, and thestages of liver fibrosis by Van Gieson staining. Exogenous antisense TIMP-1 was successfullyexpressed in vivo and could block the gene and protein expression of TIMP-1. Active and latenthepatic interstitial collagenase activities were elevated (P<0. 01), hepatic hydroxyproline contentand the accumulation of collagen types Ⅰ and Ⅲ were lowered, and liver fibrosis was alleviated inthe antisense TIMP-1 group (P <0. 01) as compared with the model group. The results demonstratethat antisense TIMP-1 recombinant plasmids have some inhibitory effect on liver fibrosis.展开更多
目的构建含有金属蛋白酶组织抑制因子-1(TIMP-1)的重组质粒并以其为模板建立荧光定量聚合酶链反应(PCR)技术检测TIMP-1的标准曲线,为荧光定量PCR准确检测TIMP-1奠定基础.方法提取大鼠星状细胞系HSC-T6的mRNA,经RT-PCR扩增TIMP-1基因后与...目的构建含有金属蛋白酶组织抑制因子-1(TIMP-1)的重组质粒并以其为模板建立荧光定量聚合酶链反应(PCR)技术检测TIMP-1的标准曲线,为荧光定量PCR准确检测TIMP-1奠定基础.方法提取大鼠星状细胞系HSC-T6的mRNA,经RT-PCR扩增TIMP-1基因后与pGMT-Vector 连接,转化到大肠杆菌DH5α,以筛选得到的阳性克隆质粒作为标准品进行梯度稀释,分别用Taqman荧光探针技术和SYBR Green I荧光染料技术进行荧光定量PCR检测,建立标准曲线,并对两者结果进行了对比.同时进行普通PCR检测,与荧光定量PCR方法检测结果进行比较.结果重组质粒经PCR扩增及序列测定,表明pGMT-TIMP-1基因已成功克隆.以不同稀释水平的标准品质粒进行荧光定量PCR扩增, 应用Taqman荧光探针技术建立的标准曲线的线性检测范围为7×104～7×108拷贝,检测灵敏度为7×104拷贝;应用SYBR Green I荧光染料技术的线性检测范围为7×106～7×108拷贝,检测灵敏度为7×106拷贝,且熔解曲线显示出现非特异性扩增;两种技术相对于普通PCR技术均具有定量功能.结论所构建的pGMT-TIMP-1基因荧光定量PCR检测标准品应用Taqman荧光探针技术建立的标准曲线线性关系好, 灵敏度和特异性高,准确可靠,此方法可作为荧光定量PCR检测TIMP-1基因的标准方法.展开更多
目的观察不同类型冠心病病人血清基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)、组织基质金属蛋白酶抑制物-1(tissue-inhibitor of metalloproteinase-1,TIMP-1)和血管紧张素Ⅱ(anginotensinⅡ, AngⅡ)的血清浓度及相关关系,探...目的观察不同类型冠心病病人血清基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)、组织基质金属蛋白酶抑制物-1(tissue-inhibitor of metalloproteinase-1,TIMP-1)和血管紧张素Ⅱ(anginotensinⅡ, AngⅡ)的血清浓度及相关关系,探讨急性冠状动脉综合征发病机制。方法冠心病病人分为急性心肌梗死组、不稳定心绞痛组(上述两组合称急性冠状动脉综合征组)和稳定心绞痛组,每组病人30例,另设健康对照组30例,比较各组间血清MMP-9、TIMP-1、MMP-9／TIMP-1和AngⅡ水平。结果急性心肌梗死组和不稳定心绞痛组血清MMP-9、MMP-9／TIMP-1和AngⅡ水平高于对照组,差异有统计学意义(P<0．01),但稳定心绞痛组血清MMP-9、TIMP-1、MMP-9／TIMP-1和AngⅡ水平与对照组比较差异无统计学意义(P> 0．05),急性冠状动脉综合征组病人血清MMP-9、MMP-9／TIMP-1与AngⅡ水平呈正相关(P<0．01)。结论血清MMP-9、MMP-9／TIMP-1和AngⅡ水平的增高与急性冠状动脉综合征相关,可作为评价冠状动脉粥样硬化斑块稳定性与病变严重程度的一个参考指标。展开更多
文摘No efficient therapy for liver fibrosis has been available. This study wasaimed to provide evidence that the introduction of a plasmid expressing antisense tissue inhibitorof metalloproteinase-1 (TIMP-1) into a rat model of immunologically induced liver fibrosis canresult in the increased activity of interstitial collagenase, thus enhancing the degradation ofcollagen. Real-time nested polymerase chain reaction ( RT-Nested-PCR) and gene recombinationtechniques were used to construct a rat antisense TIMP-1 recombinant plasmid that can be expressedin eukaryotic cells. Both the recombinant plasmid and an empty vector ( _(pc)DNA3 ) wereencapsulated with glycosyl-poly-L-lysine and injected into rats suffering from pig serum-inducedliver fibrosis. The expression of ex'ogenous transfected plasmid was assessed by Northern blot,RT-PCR, and Western blot. Hepatic interstitial collagenase activity was detected usingfluorescinisothiocyanate ( FITC)-labeledtype Ⅰ collagen. In addition to hepatic hydroxyprolinecontent, hepatic collagen types Ⅰ and Ⅲ were detected by immunohistochemical staining, and thestages of liver fibrosis by Van Gieson staining. Exogenous antisense TIMP-1 was successfullyexpressed in vivo and could block the gene and protein expression of TIMP-1. Active and latenthepatic interstitial collagenase activities were elevated (P<0. 01), hepatic hydroxyproline contentand the accumulation of collagen types Ⅰ and Ⅲ were lowered, and liver fibrosis was alleviated inthe antisense TIMP-1 group (P <0. 01) as compared with the model group. The results demonstratethat antisense TIMP-1 recombinant plasmids have some inhibitory effect on liver fibrosis.
文摘目的构建含有金属蛋白酶组织抑制因子-1(TIMP-1)的重组质粒并以其为模板建立荧光定量聚合酶链反应(PCR)技术检测TIMP-1的标准曲线,为荧光定量PCR准确检测TIMP-1奠定基础.方法提取大鼠星状细胞系HSC-T6的mRNA,经RT-PCR扩增TIMP-1基因后与pGMT-Vector 连接,转化到大肠杆菌DH5α,以筛选得到的阳性克隆质粒作为标准品进行梯度稀释,分别用Taqman荧光探针技术和SYBR Green I荧光染料技术进行荧光定量PCR检测,建立标准曲线,并对两者结果进行了对比.同时进行普通PCR检测,与荧光定量PCR方法检测结果进行比较.结果重组质粒经PCR扩增及序列测定,表明pGMT-TIMP-1基因已成功克隆.以不同稀释水平的标准品质粒进行荧光定量PCR扩增, 应用Taqman荧光探针技术建立的标准曲线的线性检测范围为7×104～7×108拷贝,检测灵敏度为7×104拷贝;应用SYBR Green I荧光染料技术的线性检测范围为7×106～7×108拷贝,检测灵敏度为7×106拷贝,且熔解曲线显示出现非特异性扩增;两种技术相对于普通PCR技术均具有定量功能.结论所构建的pGMT-TIMP-1基因荧光定量PCR检测标准品应用Taqman荧光探针技术建立的标准曲线线性关系好, 灵敏度和特异性高,准确可靠,此方法可作为荧光定量PCR检测TIMP-1基因的标准方法.
