摘要
目的探讨柔木丹颗粒对四氯化碳(CCl4)诱导的小鼠肝纤维化的治疗作用。方法将30只成年昆明小鼠随机分为对照组、肝纤维化组和柔木丹组,每组10只。肝纤维化组和柔木丹组小鼠腹腔注射含体积分数20%CCl4的橄榄油混合液5 m L·kg^(-1),对照组小鼠腹腔注射相同体积的橄榄油,每周2次,连续8周;第3周起,柔木丹组小鼠灌胃柔木丹颗粒4 g·kg^(-1),每日1次,共6周;肝纤维化组和对照组小鼠灌胃相同体积的生理盐水。实验前和末次给药后24 h(实验后)分别称各组小鼠的体质量,观察各组小鼠体质量的变化;并于取材时称小鼠肝质量,计算各组小鼠肝体比;取小鼠肝组织,进行苏木精-伊红染色和Masson染色,观察小鼠肝组织病理学变化;免疫组织化学法检测小鼠肝组织中α-平滑肌肌动蛋白(α-SMA)的定位及表达;实时聚合酶链反应检测小鼠肝组织中α-SMA、Ⅰ型胶原蛋白A1(COL1A1)、组织金属蛋白酶抑制剂1(TIMP-1)及转化生长因子-β1(TGF-β1) mRNA的表达; Western blot法检测小鼠肝组织中α-SMA、TGF-β1蛋白的表达。结果实验前3组小鼠体质量比较差异无统计学意义(F=2. 312,P> 0. 05)。肝纤维化组和柔木丹组小鼠体质量增加显著小于对照组(P <0. 01),柔木丹组小鼠体质量增加显著大于肝纤维化组(P <0. 01)。肝纤维化组小鼠肝体比显著大于对照组和柔木丹组(P <0. 01),对照组与柔木丹组小鼠肝体比比较差异无统计学意义(P> 0. 05)。肝纤维化组小鼠肝组织中α-SMA蛋白及mRNA相对表达量显著高于对照组(P <0. 01),柔木丹组小鼠肝组织中α-SMA蛋白及mRNA相对表达量显著低于肝纤维化组(P <0. 01),柔木丹组与对照组小鼠肝组织中α-SMA蛋白及mRNA相对表达量比较差异无统计学意义(P> 0. 05)。肝纤维化组小鼠肝组织中COL1A1和TIMP-1 mRNA相对表达量显著高于对照组(P <0. 01),柔木丹组小鼠肝组织中COL1A1和TIMP-1 mRNA相对表达量显著低于肝�
Objective To investigate the therapeutic effect of Roumudan granules on liver fibrosis induced by carbon tetrachloride (CCl4) in mice.Methods Thirty adult male Kunming mice were randomly divided into control group,liver fibrosis group and Roumudan granules group,with ten mice in each group.The mice in the liver fibrosis group and Roumudan granules group were intraperitoneally injected with olive oil mixture containing 20% CCl4 (5 mL·kg^-1) to establish liver fibrosis models,the mice in the control group were intraperitoneally injected with the same volume of olive oil,twice a week for 8 weeks.From the third week,the mice in the Roumudan granules group were given Roumudan granules 4 g·kg^-1 by intragastric administration,once a day for 6 weeks.The mice in the liver fibrosis group and control group were given the same volume of physiological saline by intragastric administration.The body mass of mice in each group was weighed before and 24 hours after the last administration,and the changes of body mass of mice in each group were observed.The liver mass of mice was weighed and the ratio of liver mass to body mass of mice in each group was calculated.The liver tissues of mice were taken.The pathological changes of liver tissues of mice were observed by hematoxylin-eosin staining and Mason staining.The localization and expression of α-smooth muscle actin (α-SMA) in liver tissues of mice was detected by immunohistochemical method.The expression of α-SMA,collagen type I A1 (COL1A1),tissue-inhibitor of metalloproteinase-1 (TIMP-1) and transforming growth factor-β1 (TGF-β1) mRNA in liver tissues of mice were detected by real-time polymerase chain reaction.The expression of α-SMA and TGF-β1 protein in liver tissues of mice were detected by western blot.Results There was no significant difference in the body mass of mice among the three groups before experiment (F=2.312,P >0.05).The body mass gain of mice in the liver fibrosis group and Roumudan granules group was significantly lower than that in the control group
作者
史晓燕
许小凡
宋亮
李倩
常占杰
席奇
李京涛
SHI Xiao-yan;XU Xiao-fan;SONG Liang;LI Qian;CHANG Zhan-jie;XI Qi;LI Jing-tao(Medical Experiment Center of Shaanxi University of Chinese Medicine,Xianyang 712046,Shaanxi Province,China;Department of Liver Disease,Affiliated Hospital of Shaanxi University of Chinese Medicine,Xianyang 712000,Shaanxi Province,China)
出处
《新乡医学院学报》
CAS
2019年第1期1-6,共6页
Journal of Xinxiang Medical University
基金
国家自然科学基金资助项目(编号:81704073)
陕西省中医药管理局基础面上项目(编号:JCMS030)
