摘要
目的观察脐带间充质干细胞移植对兔腹主动脉损伤后血管内膜、中膜及管腔狭窄程度的影响。方法将20只新西兰雄性大白兔分为对照组和试验组2组,每组10只,两组均采用球囊扩张损伤腹主动脉法制作兔腹主动脉损伤模型。实验组兔将1×10^7个/kg脐带间充质干细胞从球囊导管交换导丝侧孔注入兔腹主动脉血管损伤处。对照组采用同样方法注射等量生理盐水。4周后处死兔,取兔腹主动脉血管组织行石蜡切片,测量血管内膜厚度(IT)、内膜面积(IA)、血管中膜厚度(MT)及面积(MA)及狭窄程度,比较两组兔血管中膜α-平滑肌肌动蛋白(α-SMA)表达水平的差异。结果对照组IT[(1.63±0.29)mm比(0.68±0.11)mm,t=9.680,P=0.000]、IA[(4.11±1.23)mm^2比(1.42±0.56)mm^2,t=6.290,P=0.000]、MT[(2.31±0.47)mm比(1.88±0.31)mm,t=2.420,P=0.026]及MA[(6.32±1.66)mm^2比(4.02±1.06)mm^2,t=3.690,P=0.002]显著高于实验组;对照组狭窄程度(72.2±8.4)%显著高于实验组(35.0±8.6)%,差异有统计学意义(t=9.790,P=0.000);光镜下α-SMA蛋白程棕褐色颗粒,对照组阳性率为0.12±0.04显著低于实验组0.48±0.05,且差异有统计学意义(t=17.780,P=0.000)。结论人脐带间充质干细胞移植能显著降低兔腹主动脉损伤后管腔狭窄程度,并促进血管中膜α-SMA表达。
Objective The aim of this study was to evaluate the effect of human umbilical cord mesenchymal stem cell transplantation on the degree of intima, middle membrane and lumen stenosis after abdominal aorta injury in rabbits. Methods Twenty male 20 New Zealand white rabbits were divided into control group and experimental group with each 10 rabbits. The abdominal aorta injury models were made by balloon dilation in two groups. In the experimental group, the 1×10^7/kg umbilical cord mesenchymal stem cells were injected into the vascular of the abdominal aorta of the rabbit from the side hole of the balloon catheter. The control group was injected with the same amount of normal saline by the same method. 4 weeks after, the rabbit were killed, rabbit abdominal aorta intimal thickness (IT), intimal area (IA), vascular media thickness (MT) and area (MA) and the degree of stenosis were measured and compared between the two groups; The α-smooth muscle actin (α-SMA) expression in rabbit vascular smooth muscle were compared between two groups. Results The IT [(1.63±0.29) mm vs. (0.68±0.11) mm, t=9.680, P=0.0001], IA [(4.11±1.23) mm^2 vs. (1.42±0.56) mm^2,t=6.290, P=0.0001], MT [(2.31±0.47) mm vs. (1.88±0.31) mm, t=2.420, P=0.026] and MA [(6.32±1.66) mm^2 vs. (4.02±1.06) mm^2,t=3.690, P=0.0017] in experiment group were significant lower than that of control group. The degree of stenosis in the control group and the test group were (72.2±8.4)% and (35.0±8.6)% respectively with statistical difference (t=9.790, P=0.000); α-SMA was brownish brown granules under optical microscope with the positive expression rate of 0.12±0.04 and 0.48±0.05 in the control and experiment groupers respectively with statistical difference (t=17.780, P=0.0001). Conclusion Human umbilical cord mesenchymal stem cell transplantation can significantly reduce the degree of vascular stenosis after abdominal aorta injury in rabbit and promote the expression of alpha -SM-actin in the membrane.
作者
叶奎
彭秋凤
刘一东
Ye Kui;Peng Qiufeng;Liu Yidong(Department of Vascular Surgery,4th Central Hospital Affiliated to Nankai University,Tianjin 300140,China)
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2018年第12期2219-2221,共3页
Chinese Journal of Experimental Surgery
关键词
脐带间充质干细胞
腹主动脉损伤
动脉狭窄
Umbilical cord mesenchymal stem cells
Abdominal aorta injury
Arterial stenosis
