摘要
目的:探讨缺氧对肺动脉成纤维细胞(Fpa)分泌基质金属蛋白酶(MMPs)、金属蛋白酶组织抑制剂(TIMPs)的影响。方法:采用酶谱法测定Fpa培养基中MMP-2的酶活性,免疫印迹法检测培养基中MMP-2、TIMP-1的蛋白水平,免疫组化法测定细胞原位的蛋白表达,RT-PCR法检测mRNA表达量。结果:缺氧后Fpa分泌的MMP-2酶活性、细胞内外蛋白表达量、mRNA表达量均下降;而TIMP-1的表达则呈相反变化。结论:缺氧可使肺动脉成纤维细胞MMP-2/TIMP-1的表达失衡,可能参与缺氧性肺血管重建。
AIM： To investigate the effect of hypoxia on the expression of MMP- 2 and TIMP- 1 in pulmonary artery fibroblasts. METHODS： MMP- 2 activity was measured by using gelatin zymography. TIMP- 1 protein level was determined by immunocytochemistry and Western blotting, mRNA expression was determined by reverse transcriptase - polymerase chain reaction （RT- PCR）. RESULTS： The production of MMP- 2 mRNA and protein was down- regulated in pulmonary artery fibroblas/s exposed to hypoxia for 24 h. Gelatin zymography also demgnstrated that lower level of MMP - 2 activity was induced after hypoxia. Meanwhile, hypoxia induced the up - regulation of TIMP - 1 mRNA and protein in pulmonary artery fibroblasts. CONCLUSION： Hypoxia induces unbalance of MMP - 2/TIMP - 1 in pulmonary artery fibroblasts, which may contribute to the mechanism of hypoxic pulmonary vascular remodeling.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2006年第2期299-302,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.39570277)
关键词
缺氧
肺动脉
成纤维细胞
基质金属蛋白酶
金属蛋白酶1组织抑制剂
Anoxia
Pulmonary artery
Fibroblasts
Matrix metalloproteinases
Tissue - inhibitor of metalloproteinase - 1
