Objective Osteogenesis is vitally important for bone defect repair,and Zuo Gui Wan(ZGW)is a classic prescription in traditional Chinese medicine(TCM)for strengthening bones.However,the specific mechanism by which ZGW ...Objective Osteogenesis is vitally important for bone defect repair,and Zuo Gui Wan(ZGW)is a classic prescription in traditional Chinese medicine(TCM)for strengthening bones.However,the specific mechanism by which ZGW regulates osteogenesis is still unclear.The current study is based on a network pharmacology analysis to explore the potential mechanism of ZGW in promoting osteogenesis.Methods A network pharmacology analysis followed by experimental validation was applied to explore the potential mechanisms of ZGW in promoting the osteogenesis of bone marrow mesenchymal stem cells(BMSCs).Results In total,487 no-repeat targets corresponding to the bioactive components of ZGW were screened,and 175 target genes in the intersection of ZGW and osteogenesis were obtained.And 28 core target genes were then obtained from a PPI network analysis.A GO functional enrichment analysis showed that the relevant biological processes mainly involve the cellular response to chemical stress,metal ions,and lipopolysaccharide.Additionally,KEGG pathway enrichment analysis revealed that multiple signaling pathways,including the phosphatidylinositol-3-kinase/protein kinase B(PI3K/AKT)signaling pathway,were associated with ZGW-promoted osteogensis.Further experimental validation showed that ZGW could increase alkaline phosphatase(ALP)activity as well as the mRNA and protein levels of ALP,osteocalcin(OCN),and runt related transcription factor 2(Runx 2).What’s more,Western blot analysis results showed that ZGW significantly increased the protein levels of p-PI3K and p-AKT,and the increases of these protein levels significantly receded after the addition of the PI3K inhibitor LY294002.Finally,the upregulated osteogenic-related indicators were also suppressed by the addition of LY294002.Conclusion ZGW promotes the osteogenesis of BMSCs via PI3K/AKT signaling pathway.展开更多
OBJECTIVE： To evaluate the effect and safety of Zuogui pill and Yougui pill, classic Y/n and Yang tonic formula （CYYTF）, in the treatment of osteoporosis and the underlying mechanism. METHODS： Participants aged 55...OBJECTIVE： To evaluate the effect and safety of Zuogui pill and Yougui pill, classic Y/n and Yang tonic formula （CYYTF）, in the treatment of osteoporosis and the underlying mechanism. METHODS： Participants aged 55 to 75 with osteoporosis and Kidney deficiency in Traditional Chi-nese Medicine （TCM） will be included and randomly allocated into two groups： treatment group and control group. Participants in the treatment group were treated with Zuogui pill or Yougui pill TCM formula granule, while the control group received pla- cebo. Primary outcomes are the lumbar spine on bone mineral density （BMD） （L1-4） and femoral BMD. Secondary outcomes include pain intensity, health-related quality of life （HRQoL）, bone turn- over markers and safety. RESULTS： Totally 200 patients were enrolled from December 2014 to April 2016 from four hospitals. There were no statistically significant differences between the two groups at baseline （P 〉 0.05） and it was good to comparability. Statistically signifi- cant differences between the two groups were ob- served for the lumbar BMD （L1-4）, pain VAS scores and HRQoL at six months and twelve months and femoral BMD at twelve months （P 〈 0.05）, but no significant differences for femoral BMD and bone turnover markers at six months （P 〉 0.05）. More- over, significant difference was observed at differ- ent time before and after treatment in terms of lum- bar spine （L1-4） BMD, femoral BMD, pain VAS scores and health-related quality of life, and there was an crossover effect between the time and groups be- fore and after treatment. In additional, in the treat- ment group, 8 patients lost to follow-up and 3 patients had adverse events （AEs） and in the control group, 10 patients lost to follow-up and 2 patients had AEs. No remarkable differences were observed between the two groups with regard to AEs, lost rate and safety （P 〉 0.05）. CONCLUSION： Zuogui pill or Yougui pill could improve BMD, ease pain, relieve Kidney deficiencysyndr展开更多
A rapid method based on rapid resolution liquid chromatography（RRLC） coupled with a diode array detector（DAD） was developed for the simultaneous determination of six major constituents（magnoflorine,jatrorrhizine,...A rapid method based on rapid resolution liquid chromatography（RRLC） coupled with a diode array detector（DAD） was developed for the simultaneous determination of six major constituents（magnoflorine,jatrorrhizine,coptisine,palmatine,berberine and evodiamine） in traditional Chinese medicine（TCM） Zuo Jin Pill（ZJP）.The satisfactory chromatographic separation was carried on an Eclipse Plus C18 column（1.8 μm i.d.,150 mm×4.6 mm） by linear gradient elution with a mobile phase of acetonitrile-acetate buffer.All the calibration curves show good linearity（r2 0.9998）.The detection limits and quantification limits ranged in 1.4―12 ng and 4.8―30 ng,respectively.The intra-and inter-day precisions were less than 0.63% with accuracies 98.60%―100.78%,and the recoveries were from 99.45% to 100.46%.Furthermore,hierarchical cluster analysis（HCA） was used to evaluate the variation of the herbal prescription.The results demonstrate that this analytical method is simple,sensitive and reliable for rapidly analyzing six major bioactive compounds in ZJPs and is helpful to comprehensively evaluating the quality of this TCM.展开更多
Zuo Si(ca.253-ca.305)was a well-known poet in the Western Jin dynasty(265-316).More than half of his surviving poems are a series of eight“Poems on History”(Yongshi).There has been extensive research into the early ...Zuo Si(ca.253-ca.305)was a well-known poet in the Western Jin dynasty(265-316).More than half of his surviving poems are a series of eight“Poems on History”(Yongshi).There has been extensive research into the early medieval Chinese writers influenced by his“Yongshi.”However,this research can be further deepened and broadened.This article,based on previous scholarly findings,will examine the reception of these poems in three levels of literary and cultural context.The first level emphasizes the poetic practice of intertextual links between Zuo Si's poems and other literary works.The second level highlights primary sources of literary criticism to address the evaluations of Zuo Si's poems.The third level focuses on narrative to reveal how the educated elite employed these poems in their discourse.Investigating these three levels allows us to understand how poets,critics,and readers imitate,evaluate,and respond to these poems during the process of their reception.Furthermore,reception theory can help to uncover similarities and discrepancies in literary borrowings and assimilation(i.e.diction,imagery,and figure of speech)in the process of poetic composition and transmission.展开更多
树枝上,一根细棍子在移动。一只饥肠辘辘(ji chang lu lu)的广斧螳爬过来,棍子马上不动了。广斧螳爬上树枝,踩(cai)在棍子上,举着一对“大斧头”左顾右盼(zuo gu you pan)。没有肥美的蝴蝶幼(you)虫,也没有鲜嫩的蝗(huang)虫,广斧螳垂...树枝上,一根细棍子在移动。一只饥肠辘辘(ji chang lu lu)的广斧螳爬过来,棍子马上不动了。广斧螳爬上树枝,踩(cai)在棍子上,举着一对“大斧头”左顾右盼(zuo gu you pan)。没有肥美的蝴蝶幼(you)虫,也没有鲜嫩的蝗(huang)虫,广斧螳垂头丧气(chui tou sang qi)。它不知道。展开更多
文摘Objective Osteogenesis is vitally important for bone defect repair,and Zuo Gui Wan(ZGW)is a classic prescription in traditional Chinese medicine(TCM)for strengthening bones.However,the specific mechanism by which ZGW regulates osteogenesis is still unclear.The current study is based on a network pharmacology analysis to explore the potential mechanism of ZGW in promoting osteogenesis.Methods A network pharmacology analysis followed by experimental validation was applied to explore the potential mechanisms of ZGW in promoting the osteogenesis of bone marrow mesenchymal stem cells(BMSCs).Results In total,487 no-repeat targets corresponding to the bioactive components of ZGW were screened,and 175 target genes in the intersection of ZGW and osteogenesis were obtained.And 28 core target genes were then obtained from a PPI network analysis.A GO functional enrichment analysis showed that the relevant biological processes mainly involve the cellular response to chemical stress,metal ions,and lipopolysaccharide.Additionally,KEGG pathway enrichment analysis revealed that multiple signaling pathways,including the phosphatidylinositol-3-kinase/protein kinase B(PI3K/AKT)signaling pathway,were associated with ZGW-promoted osteogensis.Further experimental validation showed that ZGW could increase alkaline phosphatase(ALP)activity as well as the mRNA and protein levels of ALP,osteocalcin(OCN),and runt related transcription factor 2(Runx 2).What’s more,Western blot analysis results showed that ZGW significantly increased the protein levels of p-PI3K and p-AKT,and the increases of these protein levels significantly receded after the addition of the PI3K inhibitor LY294002.Finally,the upregulated osteogenic-related indicators were also suppressed by the addition of LY294002.Conclusion ZGW promotes the osteogenesis of BMSCs via PI3K/AKT signaling pathway.
基金Supported by the National Natural Science Foundation of China(No.81473711),(The epigenetic research of lnc RNA in Zuoguiwan Yougui Wan inducing chondrogenic differentiation of BMSCs)Special Fund for TCM Research in the Public Interest(No.201507006-01),(Balance chiropractic therapy for cervical spondylotic radiculopathy:study protocol for a randomized parallel-controlled trial)the Shaanxi Program of Science and Technology(No.2015SF072)(Clinical Study on Taibai Cream for Treating Osseous Arthritis Based on the Academic Thought of Old Country Chinese Medicine Doctor Yan-Min Li)
文摘OBJECTIVE： To evaluate the effect and safety of Zuogui pill and Yougui pill, classic Y/n and Yang tonic formula （CYYTF）, in the treatment of osteoporosis and the underlying mechanism. METHODS： Participants aged 55 to 75 with osteoporosis and Kidney deficiency in Traditional Chi-nese Medicine （TCM） will be included and randomly allocated into two groups： treatment group and control group. Participants in the treatment group were treated with Zuogui pill or Yougui pill TCM formula granule, while the control group received pla- cebo. Primary outcomes are the lumbar spine on bone mineral density （BMD） （L1-4） and femoral BMD. Secondary outcomes include pain intensity, health-related quality of life （HRQoL）, bone turn- over markers and safety. RESULTS： Totally 200 patients were enrolled from December 2014 to April 2016 from four hospitals. There were no statistically significant differences between the two groups at baseline （P 〉 0.05） and it was good to comparability. Statistically signifi- cant differences between the two groups were ob- served for the lumbar BMD （L1-4）, pain VAS scores and HRQoL at six months and twelve months and femoral BMD at twelve months （P 〈 0.05）, but no significant differences for femoral BMD and bone turnover markers at six months （P 〉 0.05）. More- over, significant difference was observed at differ- ent time before and after treatment in terms of lum- bar spine （L1-4） BMD, femoral BMD, pain VAS scores and health-related quality of life, and there was an crossover effect between the time and groups be- fore and after treatment. In additional, in the treat- ment group, 8 patients lost to follow-up and 3 patients had adverse events （AEs） and in the control group, 10 patients lost to follow-up and 2 patients had AEs. No remarkable differences were observed between the two groups with regard to AEs, lost rate and safety （P 〉 0.05）. CONCLUSION： Zuogui pill or Yougui pill could improve BMD, ease pain, relieve Kidney deficiencysyndr
基金Supported by the National Natural Science Foundation of China(No.30725045)the Special Program for New Drug Innovation of the Ministry of Science and Technology,China(Nos.2009ZX09311-001 and 2008ZX09101-Z-029)+2 种基金China Postdoctoral Science Foundation(No.20080441304)Shanghai Leading Academic Discipline Project,China(No.B906)the Scientific Foundation of Shanghai City,China(Nos.07DZ19728,09DZ1975700 and 09DZ1971500)
文摘A rapid method based on rapid resolution liquid chromatography（RRLC） coupled with a diode array detector（DAD） was developed for the simultaneous determination of six major constituents（magnoflorine,jatrorrhizine,coptisine,palmatine,berberine and evodiamine） in traditional Chinese medicine（TCM） Zuo Jin Pill（ZJP）.The satisfactory chromatographic separation was carried on an Eclipse Plus C18 column（1.8 μm i.d.,150 mm×4.6 mm） by linear gradient elution with a mobile phase of acetonitrile-acetate buffer.All the calibration curves show good linearity（r2 0.9998）.The detection limits and quantification limits ranged in 1.4―12 ng and 4.8―30 ng,respectively.The intra-and inter-day precisions were less than 0.63% with accuracies 98.60%―100.78%,and the recoveries were from 99.45% to 100.46%.Furthermore,hierarchical cluster analysis（HCA） was used to evaluate the variation of the herbal prescription.The results demonstrate that this analytical method is simple,sensitive and reliable for rapidly analyzing six major bioactive compounds in ZJPs and is helpful to comprehensively evaluating the quality of this TCM.
基金This article is made possible by a Start-up Research Grant(SRG2019-00197-FAH)from the Research Committee at the University of Macao,a visiting scholar fellowship from the CUHK-CCKF A sia-P acific Center for Chinese Studies at the Chinese University of Hong Kong,and two University of Chicago Library Travel Grants.It is based on a chapter of my doctoral dissertation,which was completed at the University of Toronto.I appreciate the comments made by Graham Sanders,J.Michael Farmer,Zhong Shulin鍾書林,Wang Xibo汪習波,Dylan Suher,and anonymous reviewers of the journal.
文摘Zuo Si(ca.253-ca.305)was a well-known poet in the Western Jin dynasty(265-316).More than half of his surviving poems are a series of eight“Poems on History”(Yongshi).There has been extensive research into the early medieval Chinese writers influenced by his“Yongshi.”However,this research can be further deepened and broadened.This article,based on previous scholarly findings,will examine the reception of these poems in three levels of literary and cultural context.The first level emphasizes the poetic practice of intertextual links between Zuo Si's poems and other literary works.The second level highlights primary sources of literary criticism to address the evaluations of Zuo Si's poems.The third level focuses on narrative to reveal how the educated elite employed these poems in their discourse.Investigating these three levels allows us to understand how poets,critics,and readers imitate,evaluate,and respond to these poems during the process of their reception.Furthermore,reception theory can help to uncover similarities and discrepancies in literary borrowings and assimilation(i.e.diction,imagery,and figure of speech)in the process of poetic composition and transmission.
文摘树枝上,一根细棍子在移动。一只饥肠辘辘(ji chang lu lu)的广斧螳爬过来,棍子马上不动了。广斧螳爬上树枝,踩(cai)在棍子上,举着一对“大斧头”左顾右盼(zuo gu you pan)。没有肥美的蝴蝶幼(you)虫,也没有鲜嫩的蝗(huang)虫,广斧螳垂头丧气(chui tou sang qi)。它不知道。
