The flower buds of Lonicera macranthoides(Shan Yin-Hua), represent an important traditional Chinese medicine and food ingredient. A phytochemical investigation of the 70% EtOH extract of the flower buds of L. macranth...The flower buds of Lonicera macranthoides(Shan Yin-Hua), represent an important traditional Chinese medicine and food ingredient. A phytochemical investigation of the 70% EtOH extract of the flower buds of L. macranthoides resulted in the isolation of 12 triterpenoids(1-12), including two new ursane-type nortriterpenes, 2α, 24-dihydroxy-23-nor-ursolic acid(1) and 2α, 4α-dihydroxy-23-nor-ursolic acid(2). Their structures were established by multiple spectroscopic methods and comparison with literature data. All isolated compounds were evaluated for their anti-inflammatory effects in LPS-activated RAW264.7 cells. Compounds 1 and 2 exhibited inhibitory effects on iNOS at the concentration of 30 μmol·L^(-1).展开更多
A 60-days feeding trial was conducted to determine the effect of dietary supplementation of Lonicera macranthoides leaf(LML) on growth performance, meat quality, and free amino acid profiles in growingfinishing pigs. ...A 60-days feeding trial was conducted to determine the effect of dietary supplementation of Lonicera macranthoides leaf(LML) on growth performance, meat quality, and free amino acid profiles in growingfinishing pigs. L. macranthoides leaves were harvested, air-dried and milled to powder. A total of 288 crossbred barrows(Large White x Landrace) with an average initial BW of 54.0 ± 3.4 kg were randomly allocated to 3 treatment groups and fed either a basal diet or a diet supplemented with 0.5% or 1.0% LML powder. Results showed that diet supplemented with LML powder did not affect average daily feed intake(ADFI) and feed: gain ratio(P> 0.05) in growing-finishing pigs. Moreover, diet supplemented with LML powder showed no significant effects on carcass traits(P > 0.05) including dressing percentage,backfat thickness and loin muscle area, and meat quality traits(P > 0.05) including meat color, intramuscular fat and cooking loss. However, diet supplemented with LML powder significantly improved free amino acid profiles in serum and longissimus thoracis muscle in growing-finishing pigs. Most importantly, dietary LML powder increased total free amino acids content(P < 0.001) and essential free amino acid content(P < 0.001) in longissimus thoracis muscle. These results indicate that LML has the potential to improve the nutritional value of meat through improving free amino acid profiles.展开更多
目的:建立同时分析测定山银花配方颗粒中3种活性成分(绿原酸、灰毡毛忍冬皂苷乙、川续断皂苷乙)的方法。方法:采用HPLC-CAD法,以乙腈为流动相A,0.4%醋酸溶液为流动相B,梯度洗脱,流速为1 m L·min^(-1);电喷雾检测器雾化温度35℃。结...目的:建立同时分析测定山银花配方颗粒中3种活性成分(绿原酸、灰毡毛忍冬皂苷乙、川续断皂苷乙)的方法。方法:采用HPLC-CAD法,以乙腈为流动相A,0.4%醋酸溶液为流动相B,梯度洗脱,流速为1 m L·min^(-1);电喷雾检测器雾化温度35℃。结果:绿原酸、灰毡毛忍冬皂苷乙、川续断皂苷乙线性范围分别为0.60~4.00μg、0.90~6.00μg、0.30~2.00μg,相关系数均达到0.999;精密度和稳定性RSD均小于2.0%;重复性RSD均小于2%;绿原酸、灰毡毛忍冬皂苷乙、川续断皂苷乙加样回收率分别为97.1%(n=6)、100.3%(n=6)、100.7%(n=6)。结论:本实验建立的方法重复性良好,准确度高,操作简单,灵敏度高,可用于山银花配方颗粒中绿原酸、灰毡毛忍冬皂苷乙、川续断皂苷乙含量测定。展开更多
基金supported by the National Natural Science Foundation of China(Nos.81602984 and 81630097)Science and Technology Planning Project of Guangdong Province(No.2015B030301005)
文摘The flower buds of Lonicera macranthoides(Shan Yin-Hua), represent an important traditional Chinese medicine and food ingredient. A phytochemical investigation of the 70% EtOH extract of the flower buds of L. macranthoides resulted in the isolation of 12 triterpenoids(1-12), including two new ursane-type nortriterpenes, 2α, 24-dihydroxy-23-nor-ursolic acid(1) and 2α, 4α-dihydroxy-23-nor-ursolic acid(2). Their structures were established by multiple spectroscopic methods and comparison with literature data. All isolated compounds were evaluated for their anti-inflammatory effects in LPS-activated RAW264.7 cells. Compounds 1 and 2 exhibited inhibitory effects on iNOS at the concentration of 30 μmol·L^(-1).
基金financial support from the Major Project of Hunan Province (2015NK1002)the National Science and Technology Support Program Funding (2012BAD39B03)Key Research and Development Program of Hunan Province(field of agricultural support) (2015NK2004, 2015JC3100)
文摘A 60-days feeding trial was conducted to determine the effect of dietary supplementation of Lonicera macranthoides leaf(LML) on growth performance, meat quality, and free amino acid profiles in growingfinishing pigs. L. macranthoides leaves were harvested, air-dried and milled to powder. A total of 288 crossbred barrows(Large White x Landrace) with an average initial BW of 54.0 ± 3.4 kg were randomly allocated to 3 treatment groups and fed either a basal diet or a diet supplemented with 0.5% or 1.0% LML powder. Results showed that diet supplemented with LML powder did not affect average daily feed intake(ADFI) and feed: gain ratio(P> 0.05) in growing-finishing pigs. Moreover, diet supplemented with LML powder showed no significant effects on carcass traits(P > 0.05) including dressing percentage,backfat thickness and loin muscle area, and meat quality traits(P > 0.05) including meat color, intramuscular fat and cooking loss. However, diet supplemented with LML powder significantly improved free amino acid profiles in serum and longissimus thoracis muscle in growing-finishing pigs. Most importantly, dietary LML powder increased total free amino acids content(P < 0.001) and essential free amino acid content(P < 0.001) in longissimus thoracis muscle. These results indicate that LML has the potential to improve the nutritional value of meat through improving free amino acid profiles.
文摘目的:建立同时分析测定山银花配方颗粒中3种活性成分(绿原酸、灰毡毛忍冬皂苷乙、川续断皂苷乙)的方法。方法:采用HPLC-CAD法,以乙腈为流动相A,0.4%醋酸溶液为流动相B,梯度洗脱,流速为1 m L·min^(-1);电喷雾检测器雾化温度35℃。结果:绿原酸、灰毡毛忍冬皂苷乙、川续断皂苷乙线性范围分别为0.60~4.00μg、0.90~6.00μg、0.30~2.00μg,相关系数均达到0.999;精密度和稳定性RSD均小于2.0%;重复性RSD均小于2%;绿原酸、灰毡毛忍冬皂苷乙、川续断皂苷乙加样回收率分别为97.1%(n=6)、100.3%(n=6)、100.7%(n=6)。结论:本实验建立的方法重复性良好,准确度高,操作简单,灵敏度高,可用于山银花配方颗粒中绿原酸、灰毡毛忍冬皂苷乙、川续断皂苷乙含量测定。
