摘要
利用金银花灰毡毛忍冬春季的茎段进行组织培养,筛选出了灰毡毛忍冬不同组培阶段适宜的培养基配方,结果认为:在初级培养阶段为A9号(MS+0.10 mg/LBA+0.10 mg/LIBA)培养基,外植体的分化率达92.50%;在继代培养阶段为B8号(MS+1.50 mg/LBA+0.05mg/L NAA)培养基,不仅灰毡毛忍冬苗生长旺盛,而且具有较高的增殖系数,增殖倍数达4倍以上;在生根培养阶段为C3号(1/2MS+1.50mg/L NAA)培养基,试管苗生根率达95%、驯化成活率达92%以上。
Using stem of honeysuckle Lonicera macranthoides growing in the spring in tissue culture,the appropriate tissue culture medium formula at different stages for Lonicera macranthoides were screened.Results showed that：in the initial training phase with the culture medium A9（MS＋ 0.10 mg/LBA＋0.10 mg/LIBA）,differential ratio of explant reaches 92.50%;in the subculture stage with the culture medium B8（MS＋1.50 mg/L BA＋0.05 mg/L NAA）,not only Lonicera macranthoides spout grews vigorously,but also with high multiplication factor over 4 times;in the rooting stage with the culture medium C3（1/2MS＋1.50 mg/LNAA）,rooting rate of test-tube plantlet reached 95%,and the survival rate of 92% after domestication.
出处
《现代农业科技》
2010年第12期101-102,106,共3页
Modern Agricultural Science and Technology
基金
湖南生物机电职业技术学院院级重点课题
关键词
金银花
灰毡毛忍冬
茎段
组织培养
honeysuckle
Lonicera macranthoides
stem
tissue culture
