目的探讨复发性口腔溃疡（ROU）患者外周血淋巴细胞的活化状态及意义.方法采用流式细胞术检测68例反复发作的口腔溃疡患者（观察组）和25例体检健康者（对照组）外周血活化淋巴细胞状态.结果观察组 NK 细胞表达的 CD16＋、CD56＋以及 T...目的探讨复发性口腔溃疡（ROU）患者外周血淋巴细胞的活化状态及意义.方法采用流式细胞术检测68例反复发作的口腔溃疡患者（观察组）和25例体检健康者（对照组）外周血活化淋巴细胞状态.结果观察组 NK 细胞表达的 CD16＋、CD56＋以及 T 细胞表达的 CD4＋、CD69＋和 CD8＋、CD69＋百分率明显高于对照组（P〈0.01）,而 B 细胞表达的 CD19＋、CD69＋的表达阳性率与对照组比差异无统计学意义（P 〉0.05）.结论采用流式细胞术检测外周血活化淋巴细胞亚群,可作为 ROU 患者临床免疫诊断的指标,具有一定的临床价值.展开更多
Purpose: To investigate whether activated B lymphocytes ( CD23 ) , activated T lymphocytes (CD25) ,macrophages(CD68) and human leucocyte antigen class II antigen (HLA- DR) were existed in epiretinal membranes (ERMs) a...Purpose: To investigate whether activated B lymphocytes ( CD23 ) , activated T lymphocytes (CD25) ,macrophages(CD68) and human leucocyte antigen class II antigen (HLA- DR) were existed in epiretinal membranes (ERMs) and subretinal membranes (SRMs) of prolif-erative intraocular disorders (PID).Methods : Twenty specimens of ERMs from rhegmatogenous retinal detachment with prolifera-tive vitreoretinopathy( PVR), traumatic PVR and secondary traction retinal detachment, and two specimens of SRMs from rhegmatogenous retinal detachment with PVR and traumatic PRV were studied using immunohistochemical staining.Results: CD68 and HLA - DR were found in all specimens, CD23 and CD25 in 4 cases of ERMs and in 1 case of SRMs, respectively.Conclusions : 1. The ERMs and SRMs of different etiology shared a common basis of inflammation and immunopathology. 2. There would be secondary cellular and humoral immunity in the ERMs and the SRMs of PID. Eye Science 1998; 14:35 - 40.展开更多
The Fas/FasL system transmits intracellular apoptotic signaling, inducing cell apoptosis. However, Fas signaling also exerts non-apoptotic functions in addition to inducing tumor cell apoptosis. For example, Fas signa...The Fas/FasL system transmits intracellular apoptotic signaling, inducing cell apoptosis. However, Fas signaling also exerts non-apoptotic functions in addition to inducing tumor cell apoptosis. For example, Fas signaling induces lung cancer tumor cells to produce prostaglandin E2 （PGE2） and recruit myeloid-derived suppressor cells （MDSCs）. Activated cytotoxic T lymphocytes （CTLs） induce and express high levels of FasL, but the effects of Fas activation initiated by FasL in CTLs on apoptosis-resistant tumor cells remain largely unclear. We purified activated CD8^＋ T cells from OT-1 mice, evaluated the regulatory effects of Fas activation on tumor cell escape and investigated the relevant mechanisms. We found that CTLs induced tumor cells to secrete PGE2 and increase tumor cell-mediated chemoattraction of MDSCs via Fas signaling, which was favorable to tumor growth. Our results indicate that CTLs may participate in the tumor immune evasion process. To the best of our knowledge, this is a novel mechanism by which CTLs play a role in tumor escape. Our findings implicate a strategy to enhance the antitumor immune response via reduction of negative immune responses to tumors promoted by CTLs through Fas signaling.展开更多
文摘目的探讨复发性口腔溃疡（ROU）患者外周血淋巴细胞的活化状态及意义.方法采用流式细胞术检测68例反复发作的口腔溃疡患者（观察组）和25例体检健康者（对照组）外周血活化淋巴细胞状态.结果观察组 NK 细胞表达的 CD16＋、CD56＋以及 T 细胞表达的 CD4＋、CD69＋和 CD8＋、CD69＋百分率明显高于对照组（P〈0.01）,而 B 细胞表达的 CD19＋、CD69＋的表达阳性率与对照组比差异无统计学意义（P 〉0.05）.结论采用流式细胞术检测外周血活化淋巴细胞亚群,可作为 ROU 患者临床免疫诊断的指标,具有一定的临床价值.
文摘Purpose: To investigate whether activated B lymphocytes ( CD23 ) , activated T lymphocytes (CD25) ,macrophages(CD68) and human leucocyte antigen class II antigen (HLA- DR) were existed in epiretinal membranes (ERMs) and subretinal membranes (SRMs) of prolif-erative intraocular disorders (PID).Methods : Twenty specimens of ERMs from rhegmatogenous retinal detachment with prolifera-tive vitreoretinopathy( PVR), traumatic PVR and secondary traction retinal detachment, and two specimens of SRMs from rhegmatogenous retinal detachment with PVR and traumatic PRV were studied using immunohistochemical staining.Results: CD68 and HLA - DR were found in all specimens, CD23 and CD25 in 4 cases of ERMs and in 1 case of SRMs, respectively.Conclusions : 1. The ERMs and SRMs of different etiology shared a common basis of inflammation and immunopathology. 2. There would be secondary cellular and humoral immunity in the ERMs and the SRMs of PID. Eye Science 1998; 14:35 - 40.
基金The work was supported by the Specialized Research Fund for the Chinese National 973 Project （2013CB530502）, the Doctoral Program of Higher Education of China （20110101110105）, the Project of the Chinese National Nature Science Foundation （31370902, 31070795, 31270944）, the Projects in Science and Technology Plan of Zhejiang Province （013C33G2010434） of China, the National Key Science and Technology Specific Proiect of China （2012ZX10002006）, the National High Technology Research and Development Program （2012AA020900）, and the Project of the Chinese National Natural Science Fund Committee for Talent Cultivation （J1103603）.
文摘The Fas/FasL system transmits intracellular apoptotic signaling, inducing cell apoptosis. However, Fas signaling also exerts non-apoptotic functions in addition to inducing tumor cell apoptosis. For example, Fas signaling induces lung cancer tumor cells to produce prostaglandin E2 （PGE2） and recruit myeloid-derived suppressor cells （MDSCs）. Activated cytotoxic T lymphocytes （CTLs） induce and express high levels of FasL, but the effects of Fas activation initiated by FasL in CTLs on apoptosis-resistant tumor cells remain largely unclear. We purified activated CD8^＋ T cells from OT-1 mice, evaluated the regulatory effects of Fas activation on tumor cell escape and investigated the relevant mechanisms. We found that CTLs induced tumor cells to secrete PGE2 and increase tumor cell-mediated chemoattraction of MDSCs via Fas signaling, which was favorable to tumor growth. Our results indicate that CTLs may participate in the tumor immune evasion process. To the best of our knowledge, this is a novel mechanism by which CTLs play a role in tumor escape. Our findings implicate a strategy to enhance the antitumor immune response via reduction of negative immune responses to tumors promoted by CTLs through Fas signaling.
