摘要
目的对比研究常见恶性肿瘤患者术前与术中淋巴细胞亚群及活性T淋巴细胞差异状况,研究手术对恶性肿瘤患者淋巴细胞亚群及其活化的影响。方法利用流式细胞仪测42例常见恶性肿瘤患者手术前、中的淋巴细胞亚群及其活性细胞。结果恶性肿瘤患者术中CD3+、CD4+、CD8+较术前有所降低,CD3+与CD4+淋巴细胞差异有统计学意义(P<0.05),与CD8+差异无统计学意义(P>0.05)。NK、CD19+、CD3+HLA-DR+明显升高,NK细胞与CD19+差异无统计学意义(P>0.05),与CD3+HLA-DR+差异有统计学意义(P<0.05)。结论恶性肿瘤患者细胞免疫功能处于抑制状态,手术使恶性肿瘤患者的细胞免疫功能进一步损害。手术、创伤等的应激反应使NK、CD19+、活性T淋巴细胞的表达上升。恶性肿瘤患者建议手术前使用免疫反应调节剂增强患者的细胞免疫功能,以确保术后的放、化疗等后续治疗的效果。
Objective To comparatively study the difference status of lymphocyte subsets and activated T lymphocyte between preoperation and intraoperation,and to investigate the influence of operation on lymphocyte subsets and their activation in the pa-tients with malignant tumors.Methods The lymphocyte subsets and their activated cells were determined in 42 cases of common malignant tumors by the flow cytometry.Results The levels of CD3 ＋ ,CD4 ＋ ,CD8 ＋ cells during intraoperation were obviously de-creased compared with preoperation,the difference in CD3 ＋ and CD4 ＋ had statistical significance（P 〈0.05）,while the difference of CD8 ＋ had no statistical significance（P 〉0.05）.NK,CD19 ＋ ,CD3 ＋ HLA-DR＋ were significantly increased,the difference of NK and CD19＋ had no statistical significance（P 〉0.05）,while the difference with CD3 ＋ HLA-DR＋ had statistical significance（P 〈0.05）. Conclusion The cellular immune function in the patients with malignant tumor is in inhibitory status.Operation makes the cellular immune function to be further injured.The stress responses of operation and trauma increase the expression of NK,CD19 and acti-vated T lymphocytes.It is suggested that the patients with malignant tumor should use the immune response modifier before opera-tion to enhance the cellular immune function for ensuring the effect of postoperative radiochemotherapy sequential treatment.
出处
《国际检验医学杂志》
CAS
2014年第20期2758-2759,共2页
International Journal of Laboratory Medicine
