摘要
目的研究ＩＬ－２－ＰＥ４０对免疫活性Ｔ细胞的影响。方法采用ＣｏｎＡ刺激的淋巴细胞增殖试验、混合淋巴细胞培养（ＭＬＣ）及细胞毒试验。结果ＩＬ－２－ＰＥ４０对ＣｏｎＡ诱导的小鼠脾细胞有十分强的细胞毒性，能选择性地抑制ＭＬＣ中抗原活化的Ｔ细胞活性，保留未活化Ｔ细胞对ＣｏｎＡ诱导的丝裂原反应，在培养ｄ３加入ＩＬ－２－ＰＥ４０比培养开始时加入对ＭＬＣ抑制作用强。结论ＩＬ－２－ＰＥ４０能够高度选择性抑制免疫活性Ｔ细胞，是ＩＬ－２Ｒ靶向治疗中具有潜力的一种免疫抑制剂。
AIM To study the effect of IL2PE 40 on activated T lymphocyte. METHODS Lymphocyte proliferation assay, mixed lymphocyte culture (MLC) and cytotoxicty assay were used. RESULTS The in vitro experiment showed that IL2PE 40 was extremely toxic for Con Astimulated spleen cells from mice,and could suppress Agactivated lymphocyte and the generation of cytotoxic T lymphocyte activity in a MLC while leaving to Con A inducible mitogenic response of the nonactivated lymphocyte. The inhibitory effect was greater when the IL2 toxin was added on day 3 as compared with day 0 of MLC. CONCLUSION Our study suggested that IL2PE 40 can selectively inhibites activated T lymphocyte, which is a potent immunosuppressive agent in IL2R targeting.
出处
《中国药理学通报》
CAS
CSCD
北大核心
1998年第1期63-65,共3页
Chinese Pharmacological Bulletin
