Objective The results of studies on association between -148C/T polymorphism in promoter region of β3-fibrinogen gene and susceptibility to cerebral infarction in Chinese population are controversial. In this study, ...Objective The results of studies on association between -148C/T polymorphism in promoter region of β3-fibrinogen gene and susceptibility to cerebral infarction in Chinese population are controversial. In this study, we summarize the results of published works in this field by a meta-analysis. Data sources Genetic association studies evaluating the β-fibdnogen gene -148C/T polymorphisms and cerebral infarction involving Chinese population published before December 2005 were collected from PubMed, EMBASE and CNKI. Study selection Case control studies involving unrelated, Han subjects aged from 18 to 80 years, and the internationally recognized diagnostic standard of cerebral infarction and genotype frequencies in control group consistent with Hardy-Weinberg equilibrium were used. Publication bias was tested by funnel plot and the odds ratios of all studies were combined dependent on the result of heterogeneity test among the individual studies. The software Review Manager （Version 4.2） was used for meta-analysis. Results Eleven studies including 1223 patients and 1433 controls met the selection criteria. There was no heterogeneity among the odds ratios （ORs） of individual studies （Х^2=17.82, P=0.06）. The combined OFt of susceptibility to cerebral infarction in -148T allele carriers compared to the wild homozygote was 1.32 （95%CI 1.12 to 1.55, P=-0.0008）. In the patients with cerebral infarction, the average plasma fibrinogen level of allele T carrier was 0.42 g/L （95% CI 0.29 to 0.54, P〈0.001）, higher than that of -148C/C homozygous ones. Conclusions β3-fibrinogen gene -148C/T polymorphism might contribute to susceptibility to cerebral infarction in Han Chinese. To reach a definitive conclusion, further gene to gene and gene to environment interactions studies on β3-fibrinogen polymorphisms and cerebral infarction with large sample size are required.展开更多
目的研究纤维蛋白原(Fg)Bβ基因多个位点的多态性与血浆水平及冠心病的关系。方法运用聚合酶链反应-限制性片段长度多态性分析技术及核苷酸序列测定鉴定FgBβ基因中7个可能与血栓相关的多态性位点:-β148C/T-、249 C/T3、45 C/T、-455G...目的研究纤维蛋白原(Fg)Bβ基因多个位点的多态性与血浆水平及冠心病的关系。方法运用聚合酶链反应-限制性片段长度多态性分析技术及核苷酸序列测定鉴定FgBβ基因中7个可能与血栓相关的多态性位点:-β148C/T-、249 C/T3、45 C/T、-455G/A-、854 G/A、1689T/G及Bc1 I G/A;比浊法测定血浆纤维蛋白原水平。结果与正常对照组相比,冠心病组血浆Fg显著升高(P<0.05);有-148C/T-、455G/A、-854G/A或Bc1 I G/A基因多态性变异组,其血浆Fg水平高于无变异组(P<0.05),其中,同时携带A-455、A-854者增高更为显著(P<0.01)。冠心病组A-455、T-148基因型频率(0.334)显著高于高血压组(0.196)和正常对照组(0.195),多态性位点G-455、C-148或A-455、T-148分别紧密连锁,符合率超过97%;Logistic回归分析发现,携带FgB-β148T-、455A基因的高血压患者,患冠心病的危险性是非携带者的1.654倍(P=0.01,95%CI:1.207-2.267)。结论FgBβ基因多态性与血浆Fg水平及缺血性心脏病发生的危险性相关;选择性FgBβ基因多态性位点的检测有助于临床上冠心病易患人群的筛查。展开更多
基金the Guangdong Science Technology Project Foundation (No. 2005B3370321)the Zhuhai Municipal Science Technology Foundation (No. PB20051015).
文摘Objective The results of studies on association between -148C/T polymorphism in promoter region of β3-fibrinogen gene and susceptibility to cerebral infarction in Chinese population are controversial. In this study, we summarize the results of published works in this field by a meta-analysis. Data sources Genetic association studies evaluating the β-fibdnogen gene -148C/T polymorphisms and cerebral infarction involving Chinese population published before December 2005 were collected from PubMed, EMBASE and CNKI. Study selection Case control studies involving unrelated, Han subjects aged from 18 to 80 years, and the internationally recognized diagnostic standard of cerebral infarction and genotype frequencies in control group consistent with Hardy-Weinberg equilibrium were used. Publication bias was tested by funnel plot and the odds ratios of all studies were combined dependent on the result of heterogeneity test among the individual studies. The software Review Manager （Version 4.2） was used for meta-analysis. Results Eleven studies including 1223 patients and 1433 controls met the selection criteria. There was no heterogeneity among the odds ratios （ORs） of individual studies （Х^2=17.82, P=0.06）. The combined OFt of susceptibility to cerebral infarction in -148T allele carriers compared to the wild homozygote was 1.32 （95%CI 1.12 to 1.55, P=-0.0008）. In the patients with cerebral infarction, the average plasma fibrinogen level of allele T carrier was 0.42 g/L （95% CI 0.29 to 0.54, P〈0.001）, higher than that of -148C/C homozygous ones. Conclusions β3-fibrinogen gene -148C/T polymorphism might contribute to susceptibility to cerebral infarction in Han Chinese. To reach a definitive conclusion, further gene to gene and gene to environment interactions studies on β3-fibrinogen polymorphisms and cerebral infarction with large sample size are required.
文摘目的研究纤维蛋白原(Fg)Bβ基因多个位点的多态性与血浆水平及冠心病的关系。方法运用聚合酶链反应-限制性片段长度多态性分析技术及核苷酸序列测定鉴定FgBβ基因中7个可能与血栓相关的多态性位点:-β148C/T-、249 C/T3、45 C/T、-455G/A-、854 G/A、1689T/G及Bc1 I G/A;比浊法测定血浆纤维蛋白原水平。结果与正常对照组相比,冠心病组血浆Fg显著升高(P<0.05);有-148C/T-、455G/A、-854G/A或Bc1 I G/A基因多态性变异组,其血浆Fg水平高于无变异组(P<0.05),其中,同时携带A-455、A-854者增高更为显著(P<0.01)。冠心病组A-455、T-148基因型频率(0.334)显著高于高血压组(0.196)和正常对照组(0.195),多态性位点G-455、C-148或A-455、T-148分别紧密连锁,符合率超过97%;Logistic回归分析发现,携带FgB-β148T-、455A基因的高血压患者,患冠心病的危险性是非携带者的1.654倍(P=0.01,95%CI:1.207-2.267)。结论FgBβ基因多态性与血浆Fg水平及缺血性心脏病发生的危险性相关;选择性FgBβ基因多态性位点的检测有助于临床上冠心病易患人群的筛查。
