摘要
目的探讨纤维蛋白原及其相关基因β148、β854多态性与冠心病的关系。方法1254例冠状动脉造影检查者,按造影结果随机分组,冠状动脉管腔狭窄≥50%者为冠心病组,共836例;冠状动脉管腔狭窄<50%者确定为对照组,共418例。取静脉血检查血脂(标准酶法)、血浆纤维蛋白原(凝血酶法)水平和肝、肾功能等。纤维蛋白原基因β148、β854多态性位点检测采用TaqManMGB探针荧光标记聚合酶链反应方法,扩增目的基因片段,然后利用荧光发光特点,经由7900HT基因分析仪进行多态位点分析。结果在1254例研究对象中,纤维蛋白原基因β148多态性位点检测成功1219例(97.2%),β854多态性位点检测成功1225例(97.7%)。与对照组比较,冠心病组年龄偏大,以男性多见,吸烟、高血压、糖尿病例次增多,甘油三酯、脂蛋白(a)和血浆纤维蛋白原均明显增高,高密度脂蛋白胆固醇明显降低(P<0.01),但总胆固醇、低密度脂蛋白胆固醇及纤维蛋白原β148、β854基因型及等位基因频率分布均无显著性差异(P>0.05)。Spearman’s相关分析发现,冠心病与纤维蛋白原β148、β854基因型之间无相关性(P>0.05),而与年龄、性别、吸烟、高血压、糖尿病、甘油三酯、高密度脂蛋白胆固醇、脂蛋白(a)和血浆纤维蛋白原之间存在显著相关性(P<0.01)。血浆纤维蛋白原与其基因β148、β854多态性之间无相关性(r=0.31,P>0.05)。结论除高血压、糖尿病等冠心病传统危险因素外,血浆纤维蛋白原与冠心病发病有明显关系,但不受其相关基因β148、β854多态性的影响。
Aim To understand the association of fibrinogen and its gene β148, β854 polymorphism with coronary heart disease. Methods 1 254 subjects were divided into coronary heart disease group （ coronary artery narrow≥50%, n=836） and control group （coronary artery narrow 〈 50%, n=418） according to coronary angiographic results. TaqMan MGB probes （FAMTM and VICR dye-labeled） polymerase chain reaction （PCR） was performed to semen the fibrinogen gene β148, β854 polymorphism in the appropriate Sequence Detection System Instruments （7900HT）. Plasma fibrinogen levels and blood hpids were simultaneously measured in the patients with coronary heart disease and controls. Plasma fibrinogen concentrations were assayed by thrombin method. Total cholesterol （TC）, triglyceride （TG） and high density lipoprotein cholesterol （HDLC} levels were assayed by standard enzyme method. Results Age, sex, smoking, hypertension, diabete mellitus, blood hpids and fibrinogen levels in coronary heart disease group were very different from that of control group （P〈0.01）. No significant difference was found in the frequencies of fibrinogen β148, β854 alleles gene and genotype between the controls and the patients with coronary heart disease （P〉0.05）. There was no significant association between fibrinogen β148, β854 genotypes and fibrinogen and the patients with coronary heart disease （P〉0.05）, but age, sex, smoking, hypertension, diabete mellitus, TG, and HDLC, lipoprotein （a） and fibrinogen levels were closely associated with coronary heart disease （P〈0.01）. Conclusions Fibrinogen levels were closely associated with coronary heart disease, but not with fibrinogen β148, β854 genotypes.
出处
《中国动脉硬化杂志》
CAS
CSCD
2005年第3期351-354,共4页
Chinese Journal of Arteriosclerosis
