摘要
目的 探讨过氧化物酶体增殖体激活受体γ2 (PPARγ2 )的Pro12Ala变异及其与肥胖或糖尿病之间的关系。方法 研究对象按BMI分为正常体重组 (91例 )与超重肥胖组 (14 0例 ) ;按WHO口服糖耐量检测标准分为糖耐量正常组 (10 3例 )、糖耐量减低组 (5 0例 )、糖尿病组 (78例 )。运用PCR以及限制性酶切鉴定方法检测PPARγ2Pro12Ala基因多态性 ,并测定血浆抗素 (Resistin)水平等指标。结果 PPARγ 2基因型Ala等位基因频率 (qA)在正常体重组为 3 85 % ,超重肥胖组为 3 93% ,基因型频率和等位基因频率在这两组之间没有显著性差异 (P >0 0 5 )。在PPARγ 2的不同基因型之间血浆抗素水平有显著性差异 (P <0 0 5 )。PPARγ 2基因Ala等位基因频率 (qA)在糖耐量正常组、糖耐量减低组与糖尿病组分别为 3 88%、4 0 0 %、3 85 % ,在这三组之间没有显著性差异 (P >0 0 5 ) ,在糖耐量正常组、糖耐量减低组、糖尿病组中PA +AA基因型者比PP基因型者血浆抗素水平要低 ,只是在糖耐量减低组PPARγ2基因Pro12Ala的不同基因型之间血浆抗素水平差异没有显著性 (P >0 0 5 )。结论 PPARγ2的Pro12Ala变异与肥胖、糖尿病无关 ,但PPARγ2的Pro12Ala变异可能与抗素表达、机体胰岛素敏感性有关。
Objective To investigate peroxisome proliferator-activated receptors γ2(PPAR γ2) polymorphism and its association with obesity or diabetes in Chinese population. Methods Normal weight group(91) and overweight and obese group(140) were included in this study, according to BMI. 103 subjects had normal glucose tolerance, 50 had impaired glucose tolerance, and 78 had diabetes mellitus, according to WHO Criteria for Diagnosis of Diabetes Mellitus. We evaluated these subjects for the Pro12Ala mutation in the PPAR gene using PCR-restriction fragment length polymorphism. Results Allele frequencies of Pro12Ala mutation of PPAR γ2 in overweight and obese subjects (qA = 3.93%) were not significantly different from those in normal weight group (qA=3.85%, P>0.05). Both in overweight and obese group and normal weight group, the genotype of PA(AA) had lower plasma resistin as compared with the genotype of PP(P<0.05). Allele frequencies of Pro12Ala were not different among Chinese subjects with normal glucose tolerance (qA =3.88%), those with impaired glucose tolerance (qA=4.00%), and those with diabetes mellitus(qA=3.85%, P>0.05). In these three groups, the genotype of PA(AA) had lower plasma resistin as compared with the genotype of PP, only in impaired glucose tolerance group the difference was not significant(P>0.05). Conclusion The Pro12Ala mutation in PPAR γ2 was not associated with either diabetes or obesity. The PPAR γ2 Ala allele may be involved in the expression of resistin and insulin sensitivity.
出处
《卫生研究》
CAS
CSCD
北大核心
2004年第3期317-320,共4页
Journal of Hygiene Research
基金
科技部科技基础性工作专项资金项目<国民营养与体质数据库>
