摘要
目的 研究视网膜母细胞瘤 (RB)抗原致敏的树突状细胞 (DC)疫苗体外特异性对RB细胞系SO RB50 杀伤作用 ,为进一步研究RB的治疗提供新的思路。方法 以RB细胞的蛋白抗原体外冲击致敏健康人 (正常对照组 )及RB患者 (患者组 )来源的成熟DC ,并将其与自体T细胞混合培养 ,诱导出抗SO RB50 的特异性细胞毒性T淋巴细胞 (CTL) ,以Burkitt淋巴瘤Raji细胞作为对照靶细胞 ,采用噻唑蓝 (MTT)分别比较CTL对SO RB50 和Raji细胞的杀伤率及正常对照组与RB患者组来源特异性杀伤率的组间差异性。结果 对SO RB50 与Raji 2种靶细胞 ,正常对照组和RB患者组的CTL细胞毒作用均随着效靶比的增加而增加 ,对SO RB50 细胞的杀伤作用各效靶比均强于相应效靶比的Raji细胞(P <0 .0 1) ;各效靶比正常对照组CTL对SO RB50 细胞的特异性杀伤作用均显著强于RB患者组CTL(P <0 .0 1) ;而各效靶比正常对照组与RB患者组CTL对Raji细胞的非特异性杀伤作用则相近 ,两者比较 ,差异无显著意义 (P >0 .0 5 )。结论 应用RB抗原负载DC可诱导出针对SO RB50 细胞抗原特异性的CTL ,这种方法对RB的治疗可能具有潜在的临床应用价值。
Objective To investigate induced antitumor immune responses by vaccination of tumor lysate-pulsed dendritic cells (DC) to kill retinoblastoma cells SO-RB_ 50 . We hope to offer new approach for the treatment of patients with retinoblastoma. Methods DC was pulsed with RB tumor lysates in vitro and incubated with autologous lymphocytes to induce antigen specific CTL (cytotoxic T lymphocyte,CTL). SO-RB_ 50 cells were used as target cells and Raji cells were used as control target cells. Cytotoxicity of CTL was evaluated by MTT method (methyl thiazolyl letrazolium). The specific cytotoxicity of CTL to SO-RB_ 50 and Raji cells was compared. The cytotoxicity of CTL from RB and normal subjects was compared between these two groups. Results Antigen specific CTL showed greater cytotoxicity to SO-RB_ 50 than Raji cells,the difference was statistically significant, P <0.01. The cytotoxicity was dose-dependent to the ratio of CTL/target cell. The nonspecific cytotoxicity to Raji cells was the same in CTL from RB patients and normal subjects,P >0.05. The specific cytotoxicity of CTL from RB patients to SO-RB_ 50 was weaker than that from the healthy subjects,P <0.01. Conclusion DC pulsed with RB tumor lysate in vitro can induce antigen specific CTL which can kill the SO-RB_ 50 target cells specifically. This method may have potential value of therapy for the RB patients. (Chin J Ophthalmol,2004,40:229-233)
出处
《中华眼科杂志》
CAS
CSCD
北大核心
2004年第4期229-233,共5页
Chinese Journal of Ophthalmology
基金
国家自然科学基金资助项目 (3 9870 80 1)
广东省自然科学基金资助项目 (980 11)
广东省科技计划基金资助项目 (2 0 0 3A3 0 2 0 3 0 2 )
"2 11"工程基金资助项目 (980 0 7)
