摘要
目的:了解三氧化二砷(AT0)对人SO-Rb50瘤细胞株在SD幼鼠前房内表达神经生长因子及血管内皮生长因子的影响。方法:出生14d的SD幼鼠10只,将含24μmol/LATO的5×108/LSO-Rb50瘤细胞悬液0.03mL注射到任1眼前房,另1眼前房注射同量不含ATO的瘤细胞悬液作为对照,每日裂隙灯观察前房肿瘤形成的情况;接种后20d,分别取出鼠前房内生长的组织测定其神经生长因子(NGF)、血管内皮生长因子(VEGF)含量,并通过统计学分析ATO的影响。结果:将含24μmol/LATO的瘤细胞悬液注射前房后,前房内出现混浊的时间平均为6d(对照组为2d),明显延长(T=9.487,P<0.01),前房内新生血管形成时间平均为13.8d(对照组为9.7d),也明显延长(T=5.212,P<0.01);无ATO干预组肿瘤组织NGF平均含量为2.1μg/L,ATO干预组为3.1μg/L,ATO干预组肿瘤组织NGF含量增加(T=3.544,P<0.05);无ATO干预组肿瘤组织VEGF平均含量为2.6μg/L,ATO干预组为0.9ng/L,ATO干预组肿瘤组织VEGF含量显著降低(T=27.8,P<0.01)。结论:ATO能够显著延长SO-Rb50瘤细胞悬液注射后前房内出现混浊的时间及新生血管形成时间,促进肿瘤组织的NGF表达,同时抑制肿瘤组织的VEGF表达。
AIM： To explore the effect of arsenic trioxide（ATO） on the growth of human SO-Rb50 tumor cells in rat anterior chamber and expression of NGF/VEGF. METHODS： The anterior chambers of one eye of ten 14- day-old SD rats were injected with 0.03mL of 5 × 10^8/L SO-Rb50 cells with 24μmol/L ATO, and the other eye were injected with the same quantity cells simply which was taken as the contrast. Then the tumor cell developing in the anterior chamber of those rats was observed by the slit lamp. 20 days after injection, the tumor tissue was taken from the 20 rats＇ eyes and examined the NGF and VEGF content. The effect of the ATO was analyzed statistically. RESULTS： After injection of oncocyte suspension with 24μmol/L ATO into the rats＇ anterior chambers, mean nepheloid time of anterior chambers was 6 days. Compared with the control group 2 days, there was significant difference（ T=9.487,P〈 0.01 ）. The mean time of anterior chamber neovascularization was 13. 8 days. Compared with the control group 9. 7 days, there was significant difference （ T = 5. 212, P 〈 0, 01 ） ; the average NGF content of the tumor tissues was 3. 1μg/L in ATO intervention group, which was more than the contrast of 2.1μg/L （T=3.544, P〈 0.05）, and the average VEGF content was 0.9ng/L in ATO intervention group, which was less than the contrast of 2.6μg/L （ T= 27.8, P〈 0.01 ）. CONCLUSION ： ATO can prolong neovascularization and turbidity time of anterior chamber after injection of SO-Rb50 oncocyte suspension, promote the expression of NGF and restrain the expression of VEGF.
出处
《国际眼科杂志》
CAS
2009年第10期1868-1870,共3页
International Eye Science
基金
中国深圳市科技计划资助项目(No.200603160)~~
