摘要
目的探讨亚砷酸全身化疗对大鼠肝移植术后肝癌复发的影响。方法采用联合免疫抑制与循环肿瘤细胞攻击大鼠肝脏建立肝移植术后肝癌复发模型,40只Sprague-Dawley大鼠经门静脉注射肝癌Walker-256细胞株并随机分成对照组和化疗组。两组术后均给予免疫抑制剂,化疗组术后同时给予亚砷酸(1mg/kg)全身化疗,观察大鼠存活和肿瘤复发情况,免疫组织化学染色法检测肝癌组织内增殖细胞核抗原(PCNA)的表达。比较两组生存时间和肿瘤肝内复发情况、荷瘤肝质量、荷瘤肝体质量比、肿瘤肝外转移及肿瘤组织中PCNA表达的差异。结果对照组和化疗组大鼠存活时间分别为(17.2±7.3)d和(28.3±5.3)d,long-rank法显示差异有统计学意义(χ2=13.06,P<0.01),肝内复发率分别为100%(20/20)和95%(19/20,χ2=1.026,P>0.05),荷瘤肝质量分别为(14.6±0.3)g和(12.7±0.3)g(t=2.205,P<0.05),荷瘤肝体质量比分别为(7.21±0.12)%和(6.24±0.09)%(t=2.440,P<0.05),肿瘤肝外转移率分别为40%(8/20)和10%(2/20,χ2=4.80,P<0.05),PCNA指数分别为(80.8±2.1)%和(61.3±1.0)%(t=3.209,P<0.05)。结论静脉使用亚砷酸对肝移植术后肝癌复发大鼠肿瘤的恶性生长及肝外转移有一定的抑制作用。
Objective To explore the influence of arsenious acid on tumor recurrence in liver transplant model rats with primary hepatocellular carcinoma.Methods Forty Sprague-Dawlay rats were injected with Walker-256 cell line via the portal vein and then were randomly divided into 2 groups:group A (immunosuppression with non-chemotherapy ) and group B(immunosuppression with arsenious acid chemotherapy).Arsenious acid chemotherapy was given 1 mg/kg.Difference in rats' survival time and intra-hepatic tumor recurrence,the weight of liver bearing cancer,the ratio of the weight of liver bearing cancer to the weight of rat,extra-hepatic metastasis,expression of proliferating cell nuclear antigen (PCNA) in tumors between the 2 groups were observed.Results The survival time were (17.2±7.3)d and (28.3±5.3)d in the groups A and B,respectively,with a significant difference (χ2=13.06,P<0.01).The intra-hepatic recurrence rate was 100%(20/20)in the group A as compared to 95%(19/20) in the group B (χ2=1.026,P>0.05);the weight of liver bearing cancer was (14.6±0.3)g as compared to (12.7±0.3)g (t=2.205,P<0.05),the ratio of the weight of liver bearing cancer to the weight of rat was (7.21±0.12)% as compared to (6.24±0.09)% (t=2.440,P<0.05),the extra-hepatic metastasis rate was 40%(8/20) as compared to 10%(2/20,χ2=4.80,P<0.05),and the PNCA index was (80.8±2.1)% as compared to (61.3±1.0)%(t=3.209,P<0.05).Conclusion Intravenous arsenious acid injection reduced the tumor growth and extra-hepatic metastasis.
出处
《中华移植杂志（电子版）》
CAS
2009年第4期302-305,共4页
Chinese Journal of Transplantation(Electronic Edition)
基金
广州省自然科学基金项目资助课题(06104600)
广东省科技计划项目资助课题(2007B031504001)
关键词
肝细胞癌
肝移植
亚砷酸盐类
复发
Hepatocellular carcinoma
Liver transplantation
Arsenites
Recurrence
