摘要
目的 :研究CyclinE、P2 7的蛋白产物在原发性胆囊癌发生、发展过程中的表达特点及相互关系。方法 :应用免疫组化S P法检测 36例原发性胆囊癌中CyclinE、P2 7的表达 ,并以同期 2 0例慢性胆囊炎作对照。结果 :①CyclinE基因蛋白产物阳性表达率为 4 7.2 % (1 7/36 ) ,显著高于其相应良性对照组的 1 0 .0 % (2 /2 0 )。P2 7蛋白表达率为 4 7.2 % (1 7/36 ) ,显著低于相应良性对照组 1 0 0 % (2 0 /2 0 )。②CyclinE在低分化、有淋巴结或远处转移表达率 (84 .6 %、6 2 .5 % )明显高于高中分化、无转移 (2 6 .1 %、1 6 .7% )。P2 7在高中分化、无淋巴结或远处转移、NevinⅠ～Ⅲ期的患者中阳性表达率 (分别为 6 0 .9%和 75 .0 % )明显高于低分化、有转移、NevinⅣ～Ⅴ期患者 (2 3.1 %、33.3% )。③P2 7与CyclinE两者呈负相关。④P2 7阳性患者生存率高于P2 7阴性患者 (P <0 .0 5 )。CyclinE阳性患者生存率低于CyclinE阴性患者 (P <0 .0 5 )。结论 :①P2 7在介导胞外信号尤其是负性信号的传导中起着极其重要的作用。在原发性胆囊癌细胞中CyclinE表达上调 ,P2 7表达下调 ,且在有淋巴结或远处转移、NevinⅣ～Ⅴ期患者中更为明显。CyclinE过度表达和P2 7表达下降 ,在胆囊癌的发生、发展、转移中起重要作用。
Objective:To investigate the expression of CyclinE?P27 in primary gallbladder carcinoma and its relation to development of the carcinoma.Methods:The expression of CyclinE?P27 in primary gallbladder carcinoma were detected by S P immunohistochemical staining,20 cases of chronic cholecystitis were collected as contrast.Results:①The positive rate of CyclinE(47.22%) in gallbladder carcinoma increased significantly,while that of P27(47.22%) decreased remarkably.②In metastasis or low differentiation there was higher expression of CyclinE. The positive rate of P27 was inversely proportional to the middle and higher differentiation group or non metastasis group.③The expression of CyclinE was inversely correlated with the expression of P27.④There was significant difference in survival time between patients with P27(+) and P27(-) or CyclinE(+) and CyclinE(-). There were statistically correlations between the first group and the second group.Conclusion:①P27 plays an important role in transmitting celluar negative signals. Once cells were stimulated by malignant signals. The expression of P27 is down regulated. There is a significant in over expression in CyclinE decreasing of P27 in development and metastasis of gallbladder carcinoma.②The detection of CyclinE?P27 may be useful in assessing the development of the cancer,judging prognosis and eventually renders a possible target for novel therapeutic strategies.
出处
《肝胆胰外科杂志》
CAS
2004年第2期90-92,共3页
Journal of Hepatopancreatobiliary Surgery
