摘要
目的 探讨过氧化物酶体增殖物活化受体γ(PPAR γ)在肝星状细胞(HSC)活化过程中的作用。方法 采用形态学、RT-PCR方法观察从正常Wistar大鼠分离的HSC在体外培养自发活化过程中以及以二甲墓亚硝胺诱导肝纤维化形成过程中HSC在体活化时PPAR γ的动态演变。结果 HSC在体外培养自发活化过程中,新鲜分离的HSC PPAR γ mRNA表达水平最高(0.71±0.01),培养第3天时即开始减低(0.48±0.03,t=19.8372,P<0.01),培养第7天后PPAR γ的表达减低70％以上,传2代时几乎检测不到。以二甲基亚硝胺诱导肝纤维化形成过程中,对照组大鼠新鲜分离的HSC表达PPAR γ(0.76±0.01),第3次注射二甲基亚硝胺后PPAR γ的表达水平显著减低(0.46±0.02,t=29.5318,P<0.01),一周末时PPAR γ表达较正常大鼠下降66％,第2周、第3周时PPAR γ几乎检测不出。结论 PPAR γ的表达参与HSC静止表型的维持,且PPAR γ表达降低是HSC由静止向活化表型转化过程的早期事件。
Objective To observe the role of PPAR γ during the activation process of hepatic stellate cells (HSC). Methods By morphology and RT-PCR, we study the changes of expression of PPAR γ in culture-activated HSC or in vivo activated HSC induced by dimethylnitrosamine (DMN). Results In vitro, the expression level of PPAR γ in freshly isolated HSC (0.72 ± 0.01) significantly reduced to 0.48 ± 0.03 on the third day of culture (t = 19.8372, P < 0.01), and reduced 70% on the seventh culture-day and could not be detected after the second passage. In vivo, HSC freshly isolated from normal control rats expressed PPAR y (0.76 ± 0.01). During the development of rat liver fibrosis induced by DMN, the expression level significantly reduced to 0.46 ± 0.02 after the third injection of DMN (t = 29.5318, P < 0.01), and reduced 66% on the end of first week and could not be detected on the end of second and third week. Conclusions The expression of PPAR γ might play an important role on the maintenance of resting-form of HSC, and the reduction of expression of PPAR γ might be an early event during the activation process of HSC.
出处
《中华肝脏病杂志》
CAS
CSCD
2004年第4期216-218,共3页
Chinese Journal of Hepatology
