摘要
目的观察过氧化物酶体增殖物激活受体-γ(PPAR-γ)的配体15-脱氧-△12,14-前列腺素J2(15d-PGJ2)对兔耳增生性瘢痕的影响,探讨15d-PGJ2防治增生性瘢痕的机制和可行性。方法选取新西兰大白兔9只,在兔耳腹侧面制作1 cm×1 cm全层皮肤缺损创面,每耳4个,共计72个,建立兔耳增生性瘢痕动物模型,随机分为实验组和对照组,分别用15d-PGJ2及生理盐水行瘢痕内注射,1次/d,连续注射7 d。停止注射后第3、6、17天取材。应用免疫组织化学方法检测CD34、转化生长因子-β1(TGF-β1)的表达。结果兔耳创面愈合后均出现不同程度类似人增生性瘢痕组织块,与对照组比较,实验组瘢痕逐渐变平变软,颜色变浅;在各时间点实验组CD34和TGF-β1的表达均较低,与对照组比较差异有统计学意义(P<0.05)。结论 PPAR-γ的配体15d-PGJ2可减少瘢痕内CD34、TGF-β1的含量,引起瘢痕萎缩,有一定的防治瘢痕增生的作用。
Objective To investigate the effect of 15-deoxy-△12,14 -prostagliandxin J2 （15d-PGJ2） on the expression of CD34 and TGF-β1 on hypertrophic scar in rabbit ears , and the possibility of 15d-PGJ2 in treatment of hy-pertrophic scar .Methods Ear hypertrophic scar model was established in 9 New Zealand white rabbits .Four wounds with total skin loses （1 cm ×1 cm） on the ventral side on each ear were constructed in all 9 rabbits.The wounds were randomly divided into the 15d-PGJ2 treatment group and control group , in which 10 μL 15d-PGJ2 and normal saline, respectively, were injected into the ear scar once a day for 7 days.On the 3rd, 6th and 17th day after the injection, 12 scars of each group were harvested .The expression of CD34 and TGF -β1 was detected by immunohistochemical method . Results Excessive dermal scars on rabbit ear that were similar to human hypertrophic scar were observed in the both two groups.Compared with control group , smaller, softer, flatter and color lighter scars were observed in treatment group .The expression of CD34 and TGF-β1 in the 15d-PGJ2 group was significantly down -regulated as compared with that in control group at different time points （P〈0.05）.Conclusion The 15d-PGJ2, ligand of PPAR-γ, can reduce the ex-pression of CD34 and TGF-β1 of hypertrophic scar in the rabbit ears and plays an important role in the prevention and treatment of hypertrophic scar .
出处
《广东医学》
CAS
CSCD
北大核心
2014年第9期1313-1316,共4页
Guangdong Medical Journal
基金
广东省医学科研基金资助项目(编号:A2011482)
广州市医药卫生科技重点项目(编号:20121A021010)
广州市医药卫生科技项目(编号:201102A213109)
