摘要
背景:乙醇中毒可致神经系统广泛、严重的损害,并可降低自然杀伤细胞应答而使细胞免疫发生变化。但有关乙醇中毒性免疫损伤和脑损伤关系的研究较少。目的:探讨慢性乙醇中毒患者血清白细胞介素-6(Interleukin-6,IL-6)和肿瘤坏死因子α(Tumornecrosisfactoralpha,TNF-α)水平变化及其与乙醇中毒性脑萎缩的关系。设计:对照实验研究。地点和对象:地点为遵义医学院附属第一医院,对象为1999-02/2001-07门诊和住院的32例慢性乙醇中毒患者,均为男性,年龄24～64岁。发病年龄24～50岁,病程2个月～6年。30例对照系健康体检者,均为男性,年龄30～60岁。干预:用双抗体夹心酶联免疫吸附试验测定血清中IL-6和TNF-α水平,并结合头颅CT扫描进行分析。主要观察指标:以脑室指数、Huckman值、侧脑室体部指数及三脑室宽度判定脑萎缩程度。根据试剂盒的校正曲线计算血清中相应的IL-6和TNF-α浓度。结果:①慢性乙醇中毒组脑萎缩发生率为50%。②慢性乙醇中毒组血清IL-6和TNF-α水平犤分别为(286.31±104.79)ng/L,(413.34±66.87)ng/L犦显著高于健康对照组犤分别为(205.43±48.67)ng/L,(261.36±51.48)ng/L犦(P<0.001)。③慢性乙醇中毒有脑萎缩者血清IL-6和TNF-α水平犤分别为(343.75±99.59)ng/L,(449.38±55.79)ng/L犦高于无脑萎?
BACKGROUND:Alcoholism can lead to wide and serious damages in nervous system a nd could reduce the responses of natural killer cells that can induce the change s in cell-mediated immunity. However,studies on the relationship between the im mune injury in alcoholism and cerebral injury are rare. OBJECTIVE:To discuss the relationship between the changes in the serum levels of interleukin-6(IL-6) and tumor necrosis factor alpha(TNF-α) and brain atro phy caused by alcoholism. DESIGN:A case-control study. SETTINGS and PARTICIPANTS:The study was completed in the First Affiliated Hosp ital of Zunyi Medical University.Thirty-two chronic alcoholism patients were se lected from outpatient and inpatient department from February 1999 to July 2001, and all of them were males aged from 24 to 64 years with an onset age from 24 to 50 years and courses of disease from 2 months to 6 years. Thirty healthy males were selected in the control group with an age from 30 to 60 years old. INTERVENTIONS:Serum levels of IL-6 and TNF-αwere detected by double-antibo dy ELISA,which was combined with cranial CT scanning for analysis. MAIN OUTCOME MEASURES:The severity of brain atrophy was determined by the inde x of ventricle, Huckman value, index of somatic part of lateral ventricle and th e width of the three ventricles.Corresponding serum IL-6 and TNF-αstrengths w ere calculated by standard curve of reagent box. RESULTS:①The incidence of brain atrophy in chronic alcoholism group was 50%. ②The serum levels of IL-6 and TNF-αin chronic alcoholism group were(286.31± 104.79)ng/L and(413.34±66.87)ng/L respectively,which were significantly higher than those of the control group[(205.43±48.67)ng/L and(261.36±51.48)ng/L respe ctively](P< 0.001).③Serum levels of IL-6 and TNF-αin chronic alcoholism with brain atrophy patients were(343.75±99.59)ng/L and(449.38±55.79)ng/L respectiv ely,which were significantly higher than those of non-brain atrophy patients[(2 28.88±75.74)ng/L and(337.31±57.96)ng/L respectively](P< 0.001).T
出处
《中国临床康复》
CAS
CSCD
2004年第13期2568-2569,共2页
Chinese Journal of Clinical Rehabilitation
基金
贵州省卫生厅立项及遵义医学院院基金资助项目(F032)~~
