摘要
目的 :观察脑脉 号口服液对高血压脑出血脑损伤的治疗作用。方法 :5 8只雄性 SD大鼠随机分为假手术组、模型组和治疗组。向双肾双夹肾血管性高血压模型大鼠右侧尾状核注入含 0 .4 U细菌胶原酶的生理盐水 2μl以诱导脑出血 ;假手术组注入等体积生理盐水。从麻醉清醒后 1h开始 ,模型组大鼠每日给蒸馏水8ml灌胃 ;治疗组给 70 kg成人剂量 6倍的脑脉 号口服液灌胃。术后 4、2 4、4 8和 72 h对各组大鼠进行神经功能缺损程度评分 ;72 h取材观察各组大鼠脑组织病理学改变 ,测定脑血肿大小、脑含水量以及 Na+和伊文思蓝(EB)含量。结果 :假手术组大鼠脑内无血肿形成 ,脑组织结构无异常 ,也未出现神经功能缺损症状。模型组大鼠可见脑内血肿形成 ,呈现明显的神经功能缺损症状 ,脑含水量、Na+ 和 EB含量较假手术组均显著增加(P<0 .0 5或 P<0 .0 1)。与模型组比较 ,治疗组大鼠神经功能缺损评分明显降低 ,脑血肿明显缩小 ,脑含水量、Na+和 EB含量均显著降低 (P<0 .0 5或 P<0 .0 1) ;脑组织病理损伤也明显改善。结论 :脑脉 号口服液通过促进脑血肿吸收 ,降低血脑屏障通透性和减轻脑水肿 ,对高血压脑出血大鼠脑损伤具有保护作用。
Objective: To investigate the protective effects and mechanisms of NaomaiⅡ oral liquor( 脑脉Ⅱ号口服液 ) on brain injury induced by hypertensive intracerebral hemorrhage (HICH) in rats. Methods: Fifty eight male SpragueDawley rats were randomly divided into shamoperation, model and treatment group. HICH was induced in the model made by clipping renal blood vessels in both kidneys and injecting 2 μl normal saline containing 0 4 U bacterial collagenase into the right caudate nucleus. Shamoperated rats were subjected to the same surgical procedures in the model rats but only 2 μl normal saline was injected into the caudate nucleus. At 1 hour after anesthesia and resuscitation, by gastric tube 8 ml distilled water was given to the model group and 6 times of NaomaiⅡ oral liquor dosage for 70 kg adult were administered to the treatment group. At 4, 24, 48, and 72 hours after operation, neurological deficits were scored. At 72 hours after operation , histopathological changes in the brain were observed, and the size of hematoma, the contents of water, sodium, and Evans blue (EB) in the brain were measured, respectively. Results: No hematoma and histological abnormalities were found in the brain, and no neurological deficits exhibited in the shamoperated rats. In rats of the model group , brain hematoma and obvious neurological deficits were seen; the contents of water, sodium and EB in the brain were markedly higher than those in the shamoperation group ( P <0 05 or P <0 01). In rats of treatment group, diameter of the hematoma was makedly reduced, neurological deficits scores were obviously decreased, the contents of water, sodium, and EB in brain were significantly attenuated ( P <0 05 or P <0 01), and the brain injury was much more alleviated in comparison with those in the model group. Conclusion: NaomaiⅡ oral liquor has protective effects on brain injury induced by HICH. It could improve absorption of hematoma , reduce permeability of bloodbrain
出处
《中国中西医结合急救杂志》
CAS
2005年第1期13-16,共4页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
广东省中医药管理局科研课题 (4 0 10 2 9)
关键词
高血压
脑出血
脑水肿
血-脑屏障
脑脉Ⅱ号口服液
hypertension
cerebral hemorrhage
brain edema
blood-brain barrier
NaomaiⅡ oral liquor
