摘要
目的:观察中药姜黄的主要活性单体成分中双脱甲氧基姜黄素(姜黄素Ⅲ)抗肿瘤血管生成作用及对内皮细胞生长的抑制作用。方法:本实验于2003-10/2004-04在解放军第三军医大学大坪医院实验动物中心(动物实验)和解放军第三军医大学西南医院烧伤研究所(细胞培养及免疫组化)完成。实验选用BALB/c裸雄性小鼠15只。建立裸小鼠移植模型后,将15只荷有A549肺腺癌的裸小鼠随机分成3个组:①阴性对照组:含60mL/L无水乙醇和60mL/L聚乙二醇400的水溶液,0.2mL/次,隔日腹腔注射1次,共8次。②阳性对照组:O-(氯乙酰甲酰)夫马菌素醇30mg/kg,0.2mL/次,隔日腹腔注射1次,共8次。③姜黄素Ⅲ组:姜黄素Ⅲ100mg/kg,0.2mL/次,隔日腹腔注射1次,共8次。治疗结束后取移植瘤组织,应用免疫组化染色检测移植瘤中CD34,标记微血管密度、血管内皮生长因子、血管内皮生长因子受体1、血管内皮生长因子受体2的表达。结果:15只小鼠全部进入结果分析。姜黄素Ⅲ组微血管密度显著少于阴性对照组犤(40.13±7.05),(76.67±10.48)条/200倍视野,P<0.01犦;姜黄素Ⅲ组血管内皮生长因子及其受体血管内皮生长因子受体1,2表达的阳性组织相对灰度值明显低于阴性对照组(1.63±0.13,1.47±0.12,1.48±0.15;2.49±0.15,1.78±0.12,2.27±0.18,P<0.01)。结论:姜黄素Ⅲ具有确切的抗人肺腺癌细胞系A549裸鼠移植瘤血管生成作用,可能与其抑制移植瘤中肿瘤细胞和/或内皮细胞血管内皮生长因子及其血管内皮生长因子受体1,2的表达有关。
AIM:To study the anti-angiogenic effect of bisdemethoxycurcumin (curcumin Ⅲ), which is the main active monome component of the Chinese herb curcumin, and investigate its inhibition to the growth of endothelial cells. METHODS: From October 2003 to April 2004, the animal experiment was carried out in the experimental animal center of Daping Hospital, Third Military Medical University of Chinese PLA, and cell culture and immunohistochemistry were completed in the Institute of Burn, Southwest Hospital, Third Military Medical University of Chinese PLA. Fifteen male BALB/c nude mice were used. After model establishment, 15 nude mice bearing human lung adenocarcinoma A549 xenografted tumor were randomly divided into 3 groups wtih 5 mice in each group: ①negative control group: The mice were treated with intraperitoneal injection of the aqueous solution containing 60 mL/L anhydrous alcohol and 60 mL/L macrogol 400, 0.2 mL for each time, once every other day for 8 times; ②positive control group: The mice were treated with intraperitoneal injection of the 30 mg/kg TNP-470, 0.2 mL for each time, once every other day for 8 times; ③curcumin Ⅲgroup: The mice were treated with intraperitoneal injection of the 100 mg/kg curcumin Ⅲ, 0.2 mL for each time, once every other day for 8 times. After treatment, the tissue of xenografted tumor was taken, and CD34 in xenografted tumor was detected and the expressions of microvessel density, vascular endothelial growth factor, vascular endothelial growth factor receptor 1 and 2 were labeled with immunohistochemical staining. RESULTS: All the 15 mice were involved in the analysis of results. The microvessel density was significantly lower in the curcumin Ⅲgroup than in the negative control group[(40.13±7.05), (76.67±10.48) strips per 200 times visual sight, P< 0.01]. The relative gray values in the positive tissues of vascular endothelial growth factor and its receptor 1 and 2 in the curcumin Ⅲgroup were obviously lower than those in the negative control group(1.63±0.13, 1
出处
《中国临床康复》
CSCD
北大核心
2005年第22期163-165,i006,共4页
Chinese Journal of Clinical Rehabilitation
