摘要
DNA克隆技术是分子生物学研究中一项重要的技术手段。自第一个质粒载体pSC1 0 1作为克隆载体以来 ,随着分子生物学技术的发展 ,克隆载体的整体结构、容载能力和转化效率都有了很大的改善。尤其是人类基因组计划的实施 ,产生了YAC和BAC克隆体系。随着植物基因组计划的进行 ,又产生了既能够克隆大片段DNA又能够将候选克隆直接通过农杆菌介导进行功能互补实验的载体。综述了几种常用大片段克隆载体YAC、BAC、BIBAC、PAC和TAC的特点及其应用 。
DNA cloning technology is the basic terrace for molecular biology.Since the first plasmid pSC101 was used in cloning DNA fragment in 1973,the structure,cloning capacity and transformation efficiency of vectors have been improved dramatically.Because of the Human Genome Project and Rice Genome Project in the early 1990s, there was a need to create high\|resolution physical maps, and the YAC, BAC, BIBAC, PAC and TAC vectors were developed.But for gene cloning,the candidate clones screened from genomic DNA library such as YAC,BAC or PAC need to be subcloned for mutant complementation.In recent years,BIBAC and TAC vectors were developed and used for both high\|resolution physical maps and Agrobacterium\|mediate\|transformation of plants.The development and their applications of the YAC,BAC,BIBAC,PAC,TAC vectors and multigene transformation system were described.
出处
《中国生物工程杂志》
CAS
CSCD
2004年第3期12-16,共5页
China Biotechnology
基金
农业部"948"项目 (2 0 0 1 2 0 3 )
国家"863"计划资助项目(2 0 0 1AA2 2 2 15 1
2 0 0 2AA2Z10 0 1 15 )
