摘要
以黏类小麦细胞质雄性不育系ms(Kots)-90-110(A)及其近等可育基因系BC5F2为材料,采用克隆测序与PCR产物直接测序方法,对黏类小麦线粒体atp6基因在花药发育各阶段的RNA编辑进行了分析。结果表明,小麦atp6基因保守区DNA序列在供试材料不育系及其近等可育基因系中完全一致,且与普通小麦和提莫菲维小麦atp6基因序列同源性为99%。两种方法测序分析atp6基因转录本保守区RNA编辑的结果规律相似。atp6基因共有15个编辑位点,其中13个发生在密码子的第一和第二位点上,这些位点的编辑都使氨基酸种类发生了变化;有2个发生在密码子的第三位点上,不引起氨基酸种类的变化;其中第6和第7位点是共转录的。随着花药发育时期的推移,各位点的编辑频率逐渐增高。不育系与其近等可育基因系相比,在引入核恢复基因后,各位点的编辑频率明显提高。编辑不充分的转录产物可能会影响线粒体功能的正常发挥,表明黏类小麦细胞质雄性不育与线粒体atp6基因转录本保守区的编辑有一定的相关性。
RNA editing of atp6 gene at different stages of pollen development was sequenced directly and from clones using the cytoplasmic male-sterile line ms（Kots）-90-110（A） and its near-isogenic line（NIL,BC5F2）.The DNA sequences of ms（Kots）-90-110（A） and the NIL had 99% identities with those of common wheat（Triticum aestivum） and T.timopheevi.There were 15 editing sites in the conservative regions of atp6 transcripts between male sterile and fertile lines,of which 13 occurred at the first or the second position of codons with alteration of amino acid type,and two occurred at the third position with no change of amino acid type.Codens at the sixth and seventh sites were cotranscripted.The frequency of the editing site was increased gradually as the develop-mental stage of anther proceeded.The restorer gene in BC5F2 obviously increased the editing frequency at each editing site.Tran-scripts that were inadequately edited might affect the normal mitochondria function.RNA editing of atp6 is probably associated with CMS in wheat with Aegilops kotschyi cytoplasm.
出处
《作物学报》
CAS
CSCD
北大核心
2010年第12期2179-2184,共6页
Acta Agronomica Sinica
基金
国家高技术研究发展计划(863计划)重大专项(2009AA101102)
国家自然科学基金项目(31071477)
高等学校博士学科点专项科研基金(20090204110024)
陕西省"13115"科技创新工程重大科技专项(2007ZDKG-020)
国家杨凌农业生物技术育种中心专项基金(99-1A)
西北农林科技大学拔尖人才支持计划项目和资助
