摘要
紫稻(Oryza sativa L.)细胞质雄性不育系紫稻A是本实验室构建的新型细胞质雄性不育系。本研究使用PCR、RT-PCR、DNA测序等技术,得到了紫稻细胞质雄性不育水稻不育系(樱香A)及其保持系(樱香B)线粒体atp6基因转录本cDNA序列。通过与基因组序列比对发现:樱香Aatp6cDNA序列中,没有发生RNA编辑;而樱香Batp6 cDNA序列中有16个编辑位点,在樱香B cDNA序列16个编辑位点位于15个密码子中,所编码的氨基酸均发生改变:在1003位点由C替换为T,导致原来编码谷氨酰胺密码子(CAA)成为终止密码子(TAA),保证atp6 mRNA编码一个正常的ATP6多肽;而由于没有发生RNA编辑,樱香A mRNA就不能翻译成正常的多肽。研究表明,RNA编辑在合成正常的ATP6多肽的过程中具有至关重要的作用,同时也说明RNA编辑可能与细胞质雄性不育相关。
Base on the of construction of a new type of cytoplasmic male sterile line Zidao A（Oryza sativa L.）,we analyzed the transcript editing of mitochondrial ATP synthase subunit 6 gene（atp6） from CMS line and its maintainer line.With PCR,RT-PCR,complete nucleotide sequences were determined for the mitochondrial atp6 gene cDNA from two lines of Purple Rice Type rice：cytoplasmic male sterile line Ying xiang A and its maintainer line Yingxiang B.The atp6 transcript of Yingxiang A had no editing site and the transcript of Ying xiang B had 16 editing sites with changes affecting 15 amino acid residues of the protein product.The editing of the atp6 transcript from Yingxiang B changed a glutamine codon（CAA） into a termination codon（TAA）,shortening the protein of Ying xiang B to the ＂standard＂ size.The Ying xiang A transcript,which had no editing site and a＂normal＂ termination codon,cannot be translated to a normal protein.The results demonstrated the important role of RNA editing in the production of the functional ATP6 subunit and suggested that RNA editing may be associated with cytoplasmic male sterility.
出处
《武汉植物学研究》
CSCD
北大核心
2010年第3期251-256,共6页
Journal of Wuhan Botanical Research
基金
国家自然科学基金(30571143)
关键词
Atp6基因
RNA编辑
细胞质雄性不育
线粒体
紫稻
Atp6 gene
Cytoplasmic male sterile（CMS）
Mitochondria
RNA editing
Zidao（Oryza sativa L.）
