摘要
DNA克隆技术是分子生物学研究中一项重要的技术手段。随着分子生物学技术的发展及植物基因组计划的进行,产生了既能够克隆大片段DNA又能够将候选基因利用农杆菌介导法进行遗传转化试验的载体。现对大片段克隆载体的发展及大片段DNA遗传转化的影响因素进行综述,并对大片段DNA遗传转化的发展作了展望。
DNA cloning technology is an important means of technology in molecular biology research. With the development of molecular biology techniques and plant genome project progresses, the vector both capable of cloning large DNA fragments and candidate genes using Agrobacterium-mediated genetic transformation experiments were produced. The development of large fragment cloning vector, and influencing factors of genetic transformation of large DNA fragments were summarized,and the development of genetic transformation of large DNA fragments were prospected in this paper.
出处
《北方园艺》
CAS
北大核心
2013年第15期208-211,共4页
Northern Horticulture
关键词
克隆载体
大片段
遗传转化
cloning vector large fragments genetic transformation
