摘要
目的 研究准分子激光原位角膜磨镶术 (laserinsitukeratomileusis ,LASIK)后角膜瓣及瓣下角膜厚度对角膜强度的影响。方法 选取成年家猫 2 8只随机分成 4个组 ,建立LASIK动物模型。A组 8只 ,制作厚度为 16 0 μm的角膜瓣 ,激光切削至剩余瓣下角膜厚度为 30 0 μm ,复位角膜瓣。B组8只 ,弃去角膜瓣 ,剩余瓣下角膜厚度为 30 0 μm。C组 6只 ,处理方法同A组 ,但剩余瓣下角膜厚度为180 μm。D组 6只 ,处理方法同B组 ,但剩余瓣下角膜厚度为 180 μm。分别于术前 ,术后 1周 ,1、2及3个月行中央角膜厚度及前房深度的测量 ,并分析角膜后表面地形图。术后 3个月行组织病理学及透射电镜检查。结果 术后 3个月时 ,A、B、C及D组前房深度增加量分别为 ( 0 16± 0 0 8)、( 0 2 1± 0 16 )、( 0 33± 0 0 9)及 ( 0 41± 0 10 )mm。A组与B组、C组与D组相比 ,差异无显著意义 (t=0 6 85 ,1 45 7;P >0 0 5 ) ;A组与C组、B组与D组相比 ,差异有显著意义 (t=3 45 8,2 5 97;P <0 0 5 )。A、B、C及D组角膜后表面前突的增加量分别为 ( 2 7 78± 9 5 2 )、( 39 5 7± 7 6 8)、( 5 8 5 7± 8 6 6 )及( 6 8 14± 19 6 9) μm。A组与B组、C组与D组相比 ,差异无显著意义 (t=2 36 1,1 0 90 ;P >0 0 5 ) ;A组与C组。
Objective To study the influence of cornea flap and residual cornea bed on cornea intensity after laser in situ keratomileusis (LASIK). Methods The established mature cats animal model of LASIK was used. In Group A, a 160 μm cornea flap was made, followed by performing ablation on the stroma by excimer laser to preserve the residual bed for 300 μm, and then the cornea flap was repositioned. The cornea flap was removed with the residual bed 300 μm remaining in Group B, while in Group C, a 160 μm cornea flap was made with the residual bed 180 μm, and followed reposition of the cornea flap. In Group D, the cornea flap was cut off and a 180 μm the residual bed was remained. Measurements included central cornea pachymetry, anterior chamber depth (ACD) and analysis of the posterior best fit sphere in cornea Topography before operation and 1 week, 1 month, 2 months and 3 months after operation. The results were analyzed statistically. 3 months after operation, the changes of cornea structures were observed by histopathological analysis and transmission electron microscope. Results Increase of ACD 3 months after operation: Group A, (0.16±0.08) mm; group B, (0.21±0.16) mm; group C, (0.33±0.09) mm; group D, (0.41±0.10) mm. There was no statistical difference between group A and group B (t=0.685,P>0.05)and between group C and group D (t=1.457,P>0.05).There was a statistical difference between group A and group C (t=3.458, P<0.05) and between group B and group D (t=2.597, P<0.05). Increase of posterior cornea prominence 3 months after operation: Group A, (27.78 ±9.52) μm; group B, (39.57±7.68) μm; group C, (58.57±8.66) μm; group D, (68.14±19.69) μm. No statistical difference was found between group A and group B (t=2.361,P>0.05) and group C and group D (t=1.090,P>0.05).There was a statistical difference between group A and group C (t=5.860, P<0.05) and between group B and group D (t=3.310, P<0.05). Light microscopy and transmission electronic microscopy showed that the arrangements of collagen
出处
《中华眼科杂志》
CAS
CSCD
北大核心
2003年第3期150-155,共6页
Chinese Journal of Ophthalmology
