摘要
在室温条件下,用0.2%、0.4%、0.8%的秋水仙素水溶液分别处理黄瓜“津绿四号”干种子和萌动种子。结果表明, 0.4%的秋水仙素浸萌动种子4 h的染色体加倍效果最好,加倍率可达26.7%。与原二倍体相比,同源四倍体的侧枝数减少,叶柄变短,叶面积增大,花器变大,果型指数减小。四倍体花粉粒大小不一,每一朵花中平均有2.3%的4孔花粉粒;四倍体花粉的可染率和萌发率均显著降低。
The dry seeds and germinating seeds of the cultivar “Jinlv No.4”of Cucumis sativus weretreated with 0.2%, 0.4% and 0.8% colchicines under room temperature, respectively. Results showed thattreatment on germinating seeds with 0.4% colchicines for 4 h yielded the highest chromosome-doubled rates(26.7%). Prominent difference occurred between induced tetraploids and diploid controls in morphology. Andthe number of lateral branches of tetraploids was less, the length of petiole was shorter, the size of the leaves andflowers was larger, and fruit index was less. The size of tetraploid pollen grains varied and nearly 2.3% four-sized pollen grains were observed in each male flower. The staining ability and germination rates of tetrap-loid pollen grains decreased greatly compared with the controls.
出处
《植物生理学通讯》
CSCD
北大核心
2004年第2期149-152,共4页
Plant Physiology Communications
基金
国家高技术研究发展计划专项经费(2002AA241251
2002AA207012)
国家自然科学基金(30170644)
教育部"跨世纪优秀人才培养计划"
教育部科技研究重点项目(01097)。
