摘要
用Oryzalin对黄瓜茎尖进行四倍体诱导试验，结果表明，浓度为100—200μmol／L Oryzalin处理24h对黄瓜茎尖离体四倍体诱导较为合适，可以有效代替秋水仙索。和秋水仙索相比，0ryzalin使用的浓度低，效果好，且毒性小，对人和环境比较安全，为诱导黄瓜染色体加倍的理想诱导剂。并用流式细胞仪对黄瓜离体诱导的幼苗进行了倍性分析试验。
The cucumber shoot apices were treated with different concentration of oryzalin or colchiine to induce tetraploid plantlet. The results showed that oryzalin was an efficient agent for induction of tetraploid cucumber with shoot apices, the suitable concentrations range was 100 - 200 μmol/L with 24 h treatment. Colchine could be efficiently replaced by oryzalin. Oryzalin concentration for tetraploid induction is very low and effective. It is low toxicity and less harmful to human and environment. Compared to colchicines, it is an ideal induction agent for cucumber chromosome doubling Flow cytometry was used to determine the ploidy of cucumber plantlets induced after oryzalin and colchine treatment in vitro.
出处
《华北农学报》
CSCD
北大核心
2006年第4期27-30,共4页
Acta Agriculturae Boreali-Sinica
基金
天津市农科院院长基金(03014)
关键词
黄瓜
胺磺灵
四倍体
染色体加倍
Cucumber
Oryzalin
Tetraploid
Chromosome doubling
