摘要
我们于1988年建立了以水稻白叶枯病菌单克隆抗体为第二抗体的酶联免疫吸附测定法,虽然具有灵敏、精确、简便、快速的优点,但用于检测水稻白叶枯病病种所带的微量细菌,灵敏度仍然不足,存在漏检现象。因而,我们对利用单克隆抗体检测水稻白叶枯病病种的方法作了进一步的改进研究。
A double antibodies sandwich ELISA with the monoclonal antibody as the second antibody for the rapid detection of Xanthomonas campestris pv.oryzae in rice seeds was developed. The bacterial from husk of the seed samples were shake-cultured in sterile liquid medium for 48 hat 25℃, and followed by ELISA detection. 126 seed samples from Jiangsu and Zhejiang were tested, 93% of seed samples from in- fected rice were positive.
出处
《中国水稻科学》
CAS
CSCD
北大核心
1992年第2期93-95,共3页
Chinese Journal of Rice Science
关键词
种子
水稻
白叶枯病
ELISA
ELISA test
McAb
seed
Xanthomonas campestris pv. oryzae
