摘要
【目的】抗螟性鉴定是转基因抗虫玉米研发的重要一环。本文主要就转基因玉米BT799对亚洲玉米螟的抗性展开评价，同时测定了BT799植株组织中CrylAc蛋白的表达量。【方法】采用了酶联免疫吸附测定法（ELISA）、室内生测和田间人工接虫鉴定3种方法。【结果】转基因抗虫玉米BT799组织中CrylAc蛋白含量分别为768．0ng／g（蛋白，鲜叶重）和1452．8～2978．5ng／g（蛋白／花丝、苞叶或幼嫩籽粒干重）。田间心叶期接虫条件下转基因抗虫玉米BT799和CC．2XBT799表现高抗亚洲玉米螟。室内取食转CrylAc基因玉米不同组织的亚洲玉米螟敏感幼虫7d后的存活率为0～37．5％，取食非转基因对照的存活率为89．9％～100．O％。亚洲玉米螟不同抗性品系取食郑单958K组织的存活率以Crylle抗性品系最低，其次是CrylF抗性品系，均显著低于取食对照郑单958，CrylAc抗性品系最高，与对照差异不显著。【结论】转CrylAc基因玉米BT799对亚洲玉米螟有很高的杀虫作用和良好的田间抗螟性。
[Objectives] To review the resistance of transgenic Bt corn to target pests which is one of the primarily steps for research and development of insect-resistant, transgenic corn. This review mainly evaluates the resistance of transgenic CrylAc gene corn variety BT799 to the Asian corn borer （ACB）, Ostrinia furnacalis （Guenee）, and measured the quantity of CrylAc protein expressed in corn plant tissues. [Methods] Enzyme-linked immunosorbent assays, laboratory bioassay and field studies with artificial infestation were employed in this study. [Results] The expression levels of CrylAc protein were 768.0 ng/g （protein/fresh leaves）, 1452.8-2978.5 ng/g （protein/dry mass of silk, husk and young kernels）. Leaf-feeding rates in field trials indicated that BT799 and CC-2XBT799 were highly resistant to ACB. The survival of ACB larvae feeding on Zhengdan 958K, a single cross hybrid containing BT799, was significantly lower （0-37.5%） than that of larvae that fed on non-Bt Zhengdan 958 （89.9%-100.0%）. In addition, larval survival of CrylAc-, Crylle-, and CrylF-selected ACB strains （ACB-AcR, ACB-IeR and ACB-FR） were significantly different when fed on Zhengdan 958K. ACB-IeR had the lowest survival followed by ACB-FR, both of them had significantly lower survival than those that fed on the control Zhengdan 958. However, the survival of ACB-AcR was not significantly different from larvae feeding on the control Zhengdan 958. [Conclusion] These results suggests that the transgenic Bt corn variety BT799 is highly toxic to the ACB and can provide effective control for ACB in the field.
出处
《应用昆虫学报》
CAS
CSCD
北大核心
2014年第3期636-642,共7页
Chinese Journal of Applied Entomology
基金
转基因作物新品种培育重大专项(2014ZX08003-001)
