摘要
目的 通过分析口腔鳞癌中浸润性树突状细胞的特征性表型 ,以探讨其在肿瘤微环境中的功能状态。方法 选择未经任何非手术治疗的初发口腔鳞癌患者标本 34例作为实验组 ,30例口腔正常粘膜组织作为对照组。通过免疫组化技术标记组织中树突状细胞 (DC)的CD1a,HLA_DR和CD83抗原 ,观察两种组织中的DC表达 3种抗原的状况 ,结果进行统计学分析。结果 所有病例均未见明显CD83+ DC ,但均有CD1a+ DC ,其在口腔鳞癌组织中的浸润程度低于正常口腔粘膜组织 ,差异有显著性 (P <0 0 5 )。在口腔鳞癌组中有 2 7例癌实质内的DC表达HLA_DR抗原 ,其HLA_DR的阳性表达率为 79 4 1%。结论 口腔鳞癌组织中的DC ,其浸润程度下降并存在功能成熟障碍。
Objective To elucidate the functional status of dendritic cells (DC) in the tissue of oral squamous cell carcinoma by analyzing characteristic phenotype of them.Methods 34 specimens from oral squamous cell carcinoma cases primarily treated with surgery were selected as test group.In addition, 30 specimens of normal mucosa from oral mucocele cases were used as control. Distribution of DC expressing CD1a++, HLA-DR++ and CD83++ in tumor tissue and normal mucous membrane was observed by immunohistochemistry. The number of DC expressing the antigens, which represented the density of DC infiltrating into tissue, was counted by microscope. The density of DC and the rate of DC expressing HLA-DR in oral carcinoma group and control were statistically compared.Results There was no CD83++ DC in all cases, but CD1a++ DC was found in all samples. The density of CD1a++ DC in tumor tissue was significantly lower than that in normal mucous membrane (P<0.05). HLA-DR antigen expressed on the surface of DC in tumoral epithelium of 27-case carcinoma specimens and in normal mucous epithelium of 23 cases. The rate of HLA-DR positive expression of TIDC had no statistic significance between the two groups.Conclusion The lower density of DC infiltrating in tumor tissue might reflect the microenviromental immunodeficiency of hosts with oral squamous cell carcinoma, and the functional mature of DC might be inhibited by the immunosuppressive action of oral squamous cell carcinoma.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2004年第2期103-105,131,共4页
West China Journal of Stomatology
