摘要
在草莓花药愈伤组织诱导及继代培养过程中 ,主要出现了 3种类型愈伤组织 :Ⅰ型愈伤组织表现为生长缓慢、淡绿色、块状或粒状 ;Ⅱ型愈伤组织表现为生长旺盛、鲜白色、松软状 ;Ⅲ型 (胚性 )愈伤组织表现为生长较旺盛、鲜黄色、松脆状。草莓花药在F +NAA 0 2mg L +BA 2 0mg L +水解酪蛋白 30 0mg L培养基上诱导的愈伤组织多为Ⅰ型 ,Ⅰ型愈伤组织在含 2 ,4 -D浓度较高 (3 0mg L)的MS培养基上培养 ,转变成Ⅱ型愈伤组织 ,Ⅱ型愈伤组织在MS +2 ,4 -D 0 5～ 1 0mg L +BA 0 0 5～ 0 1mg L +水解酪蛋白 4 0 0mg L +谷氨酰胺 15 0mg L +KCl 4 0 0mg L培养基上培养 ,提高了其质量 ,而后将质量好的Ⅱ型愈伤组织转到附加KNO3114 0mg L的MS培养基上 ,培养 4 0d后在愈伤组织块边缘出现了黄色、颗粒状愈伤组织 ,将此状态的愈伤组织在MS +2 ,4-D 1 0mg L +NAA 1 0mg L +ZT 0 2mg L +水解酪蛋白 4 0 0mg L +谷氨酰胺 15 0mg L +KCl4 0 0mg L培养基上培养获得了Ⅲ型 (胚性 )愈伤组织。Ⅰ型愈伤组织和Ⅱ型愈伤组织不适合建立细胞悬浮系 ,而Ⅲ型 (胚性 )愈伤组织很容易建立细胞悬浮系 ,并诱导出大量胚状体。Ⅰ型愈伤组织能再生植株 ,Ⅱ型愈伤组织不能再生植株 ,Ⅲ型 (胚性 )愈伤组织经状态调控成Ⅰ型愈伤组织后也能再生?
There were three types calli in the process of anther callus induction and sub-culture in strawberry. Type Ⅰ callus was growing slowly, light green, lump or granular state. Type Ⅱ callus was growing flourishly, fresh- white and swampy. Type Ⅲ(embryogenic) callus was growing more flourishly, fresh-yellow, and crisp state. Type Ⅰ callus was induced from F medium containing NAA 0.2 mg/L+BA 2.0 mg/L+CH 300 mg/L. Type Ⅱ callus was obtained from type Ⅰ callus cultured on MS medium with high 2,4-D level(3.0 mg/L). The quality of type Ⅱ callus was improved when type Ⅱ callus was sub-cultured on MS medium supplemented 2,4-D 0.5～1.0 mg/L,BA 0.05～0.1 mg/L,CH 400 mg/L,Gln 150 mg/L,KCl 400 mg/L. After the improved type Ⅱ callus being selected more than 40 d on the MS medium supplemented KNO_3 1 140 mg/L, yellow and granular callus appeared at the edge of type Ⅱ callus. Type Ⅲ(embryogenic) callus was got from yellow and granular callus on MS medium containing 2,4-D 1.0 mg/L+NAA 1.0 mg/L+ZT 0.2 mg/L+CH 400 mg/L+Gln 150 mg/L+KCl 400 mg/L. It was not suitable for type Ⅰ and type Ⅱ callus but easy for type Ⅲ(embryogenic) callus to establish cell suspension lines and lots of embryoid could be induced from suspension cell. Regeneration could be induced from type Ⅰ callus and type Ⅲ callus which was regulated to type Ⅰ callus. Type Ⅱ callus induced no regeneration.
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2004年第2期59-63,共5页
Journal of Hebei Agricultural University
