摘要
为建立稳定、高效的西北粳稻花药培养再生体系,以22个西北粳稻品种的花药为材料,N6和A4为基本培养基,研究了基因型、培养基、激素、碳源的种类和浓度对花药离体生长的影响。结果表明：基因型对西北粳稻花药培养的影响较大,参试材料中‘3279’的培养效果最好;A4培养基的培养效果优于N6;诱导愈伤组织中2,4-D浓度因基因型不同而不同,其范围在1-3 mg/L之间;花药培养以蔗糖为碳源时效果最好,而蔗糖浓度为60 g/L时培养效果最好;麦芽糖虽然有利于水稻愈伤组织的诱导,但绿苗分化率较低。
In order to establish a stable and efficient in vitro culture and regenerated system in anther of Northwest Japonica rice, 22 cultivars were studied on influence of genotype, culture medium, hormone, carbon sources and concentration using N6 culture medium and A4 culture medium. The result showed that genotype was ranked at the top of the factors, ＇3279＇ was high culture abili- ty. Using N6 culture medium and A4 culture medium, A4 culture medium was better than N6 cul- ture medium. Different genotypes had different culture abilities for concentration of 2,4 - D, the extent was 1 - 3 mg / L. Sucrose was better to induce callus using anther, and 60 g/L was the best for proportion of sucrose to induce and differentiate callus. Although maltose was better to induce callus, green plantlet differentiation frequency was low.
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2009年第4期8-13,共6页
Journal of Hebei Agricultural University
基金
宁夏自然科学基金项目(NZ0756)
宁夏粮食增产技术研究与示范项目-水稻高产节水新品种选育
关键词
粳稻
花药
愈伤组织
再生体系
Japonica rice
anther
callus
regeneration system
